Banaee Mahdi, Zeidi Amir, Gholamhosseini Amin, Shakeri Reza, Faggio Caterina, Multisanti Cristiana Roberta
Aquaculture Department, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Division of Aquatic Animal Health & Diseases, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Ecotoxicology. 2025 Jul;34(5):769-778. doi: 10.1007/s10646-025-02872-1. Epub 2025 Mar 21.
The present study aimed to evaluate the effects of microplastics (MPs) and Zinc Oxide nanoparticles (ZnO-NPs), both individually and in combination, on the crayfish, Astacus leptodactylus. A total of 360 crayfish were assigned to eight experimental groups, including control and treatment groups exposed to MPs (polyethylene, polystyrene, and polyvinyl chloride at 300 µg/L) and ZnO-NPs (5 µg/L), individually and combined. Biochemical analyses on the haemolymph revealed a significant increase in aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamic transpeptidase activities, and glucose and cholesterol levels in crayfish exposed to MPs and ZnO-NPs. In contrast, butyrylcholinesterase activity was significantly decreased in all treatments compared to the control. Lactate dehydrogenase activity increased significantly in crayfish exposed to ZnO-NPs, alone or combined with MPs. Furthermore, thiol groups, total antioxidant levels, and glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase were significantly decreased, while malondialdehyde levels were increased. The integrated biomarker response (IBR) and radar plots highlighted a cumulative oxidative stress response, suggesting potential synergistic or additive interactions between MPs and ZnO-NPs.
本研究旨在评估微塑料（MPs）和氧化锌纳米颗粒（ZnO-NPs）单独及联合作用对小龙虾（Astacus leptodactylus）的影响。总共360只小龙虾被分配到八个实验组，包括对照组以及分别暴露于MPs（聚乙烯、聚苯乙烯和聚氯乙烯，浓度为300 μg/L）和ZnO-NPs（5 μg/L）的处理组，还有MPs与ZnO-NPs联合暴露的处理组。对血淋巴的生化分析显示，暴露于MPs和ZnO-NPs的小龙虾体内天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、γ-谷氨酰转肽酶活性以及葡萄糖和胆固醇水平显著升高。相反，与对照组相比，所有处理组中的丁酰胆碱酯酶活性均显著降低。单独暴露于ZnO-NPs或同时暴露于MPs与ZnO-NPs的小龙虾体内乳酸脱氢酶活性显著增加。此外，巯基、总抗氧化水平以及谷胱甘肽过氧化物酶、谷胱甘肽还原酶、过氧化氢酶和超氧化物歧化酶水平均显著降低，而丙二醛水平升高。综合生物标志物响应（IBR）和雷达图突出显示了累积的氧化应激反应，表明MPs和ZnO-NPs之间可能存在协同或相加相互作用。
