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虫草素通过激活SLC7A11/GPX4通路改善糖尿病肾病损伤。

Cordycepin ameliorates diabetic nephropathy injury by activating the SLC7A11/GPX4 pathway.

作者信息

Wu Bing, Wang Jing, Yan Xiaohui, Jin Gang, Wang Qiong

机构信息

Department of Nephrology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China.

出版信息

J Diabetes Investig. 2025 Jun;16(6):992-1000. doi: 10.1111/jdi.14407. Epub 2025 Mar 22.

DOI:10.1111/jdi.14407
PMID:40120097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12131933/
Abstract

BACKGROUND

Cordycepin (CRD) has been identified to alleviate diabetes-induced injuries and complications including diabetic nephropathy (DN). Here, this work focused on probing the specific effects and potential mechanisms of CRD on DN progression.

METHODS

High glucose (HG)-induced mouse podocyte cell line (MPC5) was used for in vitro functional analyses. Cell proliferation and apoptosis were determined using cell counting kit-8 assay, 5-ethynyl-2'-deoxyuridine assay, and flow cytometry, respectively. ELISA analysis detected inflammatory factors. Cell ferroptosis was assessed by measuring the levels of Fe2+, glutathione, reactive oxygen species, and malonaldehyde.

RESULTS

CRD treatment suppressed HG-induced apoptosis, inflammation, and ferroptosis in podocytes. CRD treatment elevated SLC7A11 and GPX4 expression in HG-treated podocytes. The overexpression of SLC7A11 or GPX4 suppressed HG-evoked apoptosis, inflammation, and ferroptosis in podocytes. Moreover, the silencing of SLC7A11 or GPX4 abolished the protective effects of CRD on HG-treated podocytes. Moreover, CRD ameliorated renal structure injury and inflammation in STZ-induced diabetic mice by modulating SLC7A11 or GPX4 expression.

CONCLUSIONS

Cordycepin suppressed HG-induced apoptosis, inflammation, and ferroptosis in podocytes in vitro, and ameliorated renal injury and inflammation in STZ-induced diabetic mice by activating the SLC7A11/GPX4 pathway.

摘要

背景

已证实虫草素（CRD）可减轻糖尿病引起的损伤和并发症，包括糖尿病肾病（DN）。在此，本研究着重探讨CRD对DN进展的具体作用及潜在机制。

方法

采用高糖（HG）诱导的小鼠足细胞系（MPC5）进行体外功能分析。分别使用细胞计数试剂盒-8法、5-乙炔基-2'-脱氧尿苷法和流式细胞术测定细胞增殖和凋亡。酶联免疫吸附测定（ELISA）分析检测炎症因子。通过测量Fe2+、谷胱甘肽、活性氧和丙二醛水平评估细胞铁死亡。

结果

CRD处理可抑制HG诱导的足细胞凋亡、炎症和铁死亡。CRD处理可提高HG处理的足细胞中SLC7A11和GPX4的表达。SLC7A11或GPX4的过表达可抑制HG诱导的足细胞凋亡、炎症和铁死亡。此外，沉默SLC7A11或GPX4可消除CRD对HG处理的足细胞的保护作用。此外，CRD通过调节SLC7A11或GPX4的表达改善链脲佐菌素诱导的糖尿病小鼠的肾脏结构损伤和炎症。

结论

虫草素在体外抑制HG诱导的足细胞凋亡、炎症和铁死亡，并通过激活SLC7A11/GPX4途径改善链脲佐菌素诱导的糖尿病小鼠的肾损伤和炎症。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/3c1ed901241e/JDI-16-992-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/80caabd68add/JDI-16-992-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/e494f56566aa/JDI-16-992-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/e4d9e03e0d7a/JDI-16-992-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/f901eae06bd1/JDI-16-992-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/3c1ed901241e/JDI-16-992-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/80caabd68add/JDI-16-992-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/e494f56566aa/JDI-16-992-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/e4d9e03e0d7a/JDI-16-992-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/f901eae06bd1/JDI-16-992-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d40/12131933/3c1ed901241e/JDI-16-992-g004.jpg

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本文引用的文献

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Sci Rep. 2024 May 21;14(1):11591. doi: 10.1038/s41598-024-62547-5.
2
VDR Activation Attenuates Renal Tubular Epithelial Cell Ferroptosis by Regulating Nrf2/HO-1 Signaling Pathway in Diabetic Nephropathy.维生素D受体激活通过调节糖尿病肾病中Nrf2/HO-1信号通路减轻肾小管上皮细胞铁死亡
Adv Sci (Weinh). 2024 Mar;11(10):e2305563. doi: 10.1002/advs.202305563. Epub 2023 Dec 25.
3
Podocyte injury of diabetic nephropathy: Novel mechanism discovery and therapeutic prospects.
糖尿病肾病足细胞损伤：新机制发现与治疗前景。
Biomed Pharmacother. 2023 Dec;168:115670. doi: 10.1016/j.biopha.2023.115670. Epub 2023 Oct 13.
4
Cordycepin from Cordyceps militaris ameliorates diabetic nephropathy via the miR-193b-5p/MCL-1 axis.来自蛹虫草的虫草素通过miR-193b-5p/MCL-1轴改善糖尿病肾病。
Chin Med. 2023 Oct 13;18(1):134. doi: 10.1186/s13020-023-00842-5.
5
Emodin attenuates diabetic kidney disease by inhibiting ferroptosis via upregulating Nrf2 expression.大黄素通过上调 Nrf2 表达抑制铁死亡来减轻糖尿病肾病。
Aging (Albany NY). 2023 Aug 7;15(15):7673-7688. doi: 10.18632/aging.204933.
6
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