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一种适用于飞行时间二次离子质谱（ToF-SIMS）单细胞成像中冷冻干燥样品制备的实验室友好型方案。

A laboratory-friendly protocol for freeze-drying sample preparation in ToF-SIMS single-cell imaging.

作者信息

Shi Xiujuan, Liu Mingru, Qi Yue, Ma Hongzhe, Wang Zhaoying, Chen Yanhua, Abliz Zeper

机构信息

Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Center for Imaging and Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing, China.

Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing, China.

出版信息

Front Chem. 2025 Mar 7;13:1523712. doi: 10.3389/fchem.2025.1523712. eCollection 2025.

DOI:10.3389/fchem.2025.1523712
PMID:40124708
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11925917/
Abstract

ToF-SIMS is a high spatial resolution imaging technique for cellular or subcellular analysis of biological samples. Accurate molecular data in single-cell studies depend on proper cell morphology and chemical integrity, highlighting the importance of sample preparation. In this work, we standardized a more efficient freeze-drying method using standard lab materials and improved the sample preparation process. Our comprehensive freeze-drying protocol for cellular samples, encompassing washing, fixation, and drying steps, facilitates the acquisition of enhanced cellular information and ensures high reproducibility. These improvements are poised to significantly advance single-cell mass spectrometry imaging research.

摘要

飞行时间二次离子质谱（ToF-SIMS）是一种用于对生物样品进行细胞或亚细胞分析的高空间分辨率成像技术。单细胞研究中的准确分子数据取决于适当的细胞形态和化学完整性，这凸显了样品制备的重要性。在这项工作中，我们使用标准实验室材料规范了一种更高效的冷冻干燥方法，并改进了样品制备过程。我们针对细胞样品的综合冷冻干燥方案，包括洗涤、固定和干燥步骤，有助于获取更多的细胞信息并确保高重现性。这些改进有望显著推动单细胞质谱成像研究的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/bd55745379de/fchem-13-1523712-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/90921f3741b6/fchem-13-1523712-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/e766c0e546c0/fchem-13-1523712-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/de62ecbf1ca6/fchem-13-1523712-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/64cf0d70fd94/fchem-13-1523712-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/39281a1e0206/fchem-13-1523712-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/5cf93e10670f/fchem-13-1523712-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/bd55745379de/fchem-13-1523712-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/90921f3741b6/fchem-13-1523712-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/e766c0e546c0/fchem-13-1523712-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/de62ecbf1ca6/fchem-13-1523712-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/64cf0d70fd94/fchem-13-1523712-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/39281a1e0206/fchem-13-1523712-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/5cf93e10670f/fchem-13-1523712-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a37f/11925917/bd55745379de/fchem-13-1523712-g007.jpg

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Mass spectrometry imaging for spatially resolved multi-omics molecular mapping.用于空间分辨多组学分子图谱的质谱成像
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Multiscale biochemical mapping of the brain through deep-learning-enhanced high-throughput mass spectrometry.
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Advancements in ToF-SIMS imaging for life sciences.用于生命科学的飞行时间二次离子质谱成像技术的进展。
Front Chem. 2023 Aug 24;11:1237408. doi: 10.3389/fchem.2023.1237408. eCollection 2023.
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Recent advances in mass spectrometry imaging of single cells.单细胞质谱成像技术的最新进展。
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Mass Spectrometry Imaging for Single-Cell or Subcellular Lipidomics: A Review of Recent Advancements and Future Development.质谱成像技术在单细胞或亚细胞脂质组学中的应用：综述最新进展与未来发展
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Advances in Mass Spectrometry-Based Single Cell Analysis.基于质谱的单细胞分析进展。
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