Wang Yufeng, Zhang Xiaokang, Shi Peijun, Zhao Wei, Wang Bin, Zhang Qiang
Key Laboratory of Advanced Design and Intelligent Computing, Ministry of Education, School of Software Engineering, Dalian University, Dalian 116622, China.
School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China.
Nanoscale. 2025 Apr 10;17(15):9502-9514. doi: 10.1039/d4nr05369a.
The rapid development of DNA nanotechnology has made it possible to explore information security methods based on non-computational complexity, providing an effective way to avoid the threats that high-performance computational methods pose to modern cryptography. However, most molecular information security methods require both external stimuli and specific DNA signals, placing high demands for experimental conditions and DNA-sequence design, limiting their practical application and further development. Herein, we proposed an EHairpin-driven double-stem-loop programmable allosteric strategy for molecular security access control. Specifically, this strategy regulates the conformational changes in the double-stem-loop programmatically by responding to specific DNA input signals, converting molecular conformational changes into signal-response triggering events. We constructed a programmable allosteric strategy through the EHairpin structure to achieve the temporal response of the DNA signal-driven molecular structure and further built a molecular-switch-response circuit for multiple input signals. Finally, we implemented an EHairpin-driven molecular security access control system, which has a three-level security assurance mechanism of administrator authentication, authorization, and user authentication. This strategy offers a powerful method for security access control of molecular devices, further promoting the development of next-generation information security and providing some new ideas for the secure control of nanomachines, which has great potential in biosensing and disease diagnosis.
DNA纳米技术的快速发展使得探索基于非计算复杂性的信息安全方法成为可能，为避免高性能计算方法对现代密码学构成的威胁提供了一条有效途径。然而，大多数分子信息安全方法既需要外部刺激又需要特定的DNA信号，这对实验条件和DNA序列设计提出了很高的要求，限制了它们的实际应用和进一步发展。在此，我们提出了一种用于分子安全访问控制的E发夹驱动的双茎环可编程变构策略。具体而言，该策略通过响应特定的DNA输入信号以可编程方式调节双茎环中的构象变化，将分子构象变化转化为信号响应触发事件。我们通过E发夹结构构建了一种可编程变构策略，以实现DNA信号驱动的分子结构的时间响应，并进一步构建了用于多个输入信号的分子开关响应电路。最后，我们实现了一个E发夹驱动的分子安全访问控制系统，该系统具有管理员认证、授权和用户认证的三级安全保证机制。该策略为分子设备的安全访问控制提供了一种强大的方法，进一步推动了下一代信息安全的发展，并为纳米机器的安全控制提供了一些新的思路，在生物传感和疾病诊断方面具有巨大潜力。
