Chen Wangbo, Xu Sun, Zou Shuzhen, Liu Zijian, Liu Yichi, Xu Haozhe, Wang Jiayue, Ma Junjie, Chen Rong, Zuo Zhaojiang
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, 311300, China.
Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-Based Healthcare Functions, Zhejiang A&F University, Hangzhou, 311300, China.
Biotechnol Biofuels Bioprod. 2025 Mar 24;18(1):36. doi: 10.1186/s13068-025-02615-8.
Biofuels produced from algae have enormous advantages in replacing fossil fuels, and Microcystis aeruginosa has a great potential for biofuel production, due to growing fast to form large amounts of biomass. Light is essential for algal growth, and the optimum light quality can promote the biomass and lipid accumulation for increasing feedstock for biofuel production.
We investigated the biomass accumulation, photosynthetic ability, carbohydrate, and lipid yield as well as related gene expression in M. aeruginosa under red, blue, purple, and white light to promote biofuel production using this alga under the optimal light quality. Compared with white light, purple light promoted the cell growth during the 5 days, while blue light showed inhibitory effect. Red light had no effect on the cell growth, but improved the biomass content to the highest level. Red light improved the photosynthetic ability by raising chlorophyll level, and up-regulating expression of the genes in chlorophyll biosynthesis, photosynthetic electron transfer, and CO fixation. Among these light qualities, red light showed the maximum effect on soluble, insoluble, and total carbohydrate accumulation by up-regulating the genes in polysaccharide and starch formation, and down-regulating the genes in glycolysis and tricarboxylic acid cycle. Red light also exhibited the maximum effect on lipid accumulation, which might be caused by up-regulating five genes in fatty acid biosynthesis.
Red light can promote M. aeruginosa accumulating carbohydrates and lipids by regulating related gene expression, which should be the optimal light quality for improving feedstock yield for biofuel production.
藻类生产的生物燃料在替代化石燃料方面具有巨大优势，铜绿微囊藻生长迅速，能形成大量生物质，在生物燃料生产方面具有很大潜力。光照对藻类生长至关重要，适宜的光质可促进生物质和脂质积累，从而增加生物燃料生产的原料。
我们研究了在红光、蓝光、紫光和白光下铜绿微囊藻的生物质积累、光合能力、碳水化合物和脂质产量以及相关基因表达，以在最佳光质条件下利用这种藻类促进生物燃料生产。与白光相比，紫光在5天内促进了细胞生长，而蓝光表现出抑制作用。红光对细胞生长无影响，但将生物质含量提高到了最高水平。红光通过提高叶绿素水平以及上调叶绿素生物合成、光合电子传递和碳固定相关基因的表达来提高光合能力。在这些光质中，红光通过上调多糖和淀粉形成相关基因以及下调糖酵解和三羧酸循环相关基因，对可溶性、不溶性和总碳水化合物积累产生的影响最大。红光对脂质积累的影响也最大，这可能是由于上调了脂肪酸生物合成中的五个基因所致。
红光可通过调节相关基因表达促进铜绿微囊藻积累碳水化合物和脂质，这应是提高生物燃料生产原料产量的最佳光质。
