Guo Suqin, Zhang Fa, Peng Feng, Yu Haixia, Zhang Kun, Tan Guiyu
School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.
Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Sci Prog. 2025 Jan-Mar;108(1):368504251325630. doi: 10.1177/00368504251325630. Epub 2025 Mar 25.
is an endemic plant of southern China, which is the primary country for its production. Trans-anethole (-anethole) is the key component of the volatile aromatic essence in , and it has therapeutic effects such as anti-cancer and anti-diabetes. However, its biosynthetic pathway in is rarely reported.
In the present study, we cloned and expressed the cDNA encoding -anol/isoeugenol synthase (IvAIS1) in . The characteristics of the IvAIS1 were determined and its gene expression in different tissues was measured by real-time polymerase chain reaction.
The IvAIS1 protein is 76% identical to isoeugenol synthase, and the two proteins were clustered closely together in the clade of IGS and EGS. IvAIS1 exhibits NADPH-dependent enzyme activity and dual product specificity, and it converts coumaryl acetate and coniferyl acetate to -anol (the precursor of -anethole) and isoeugenol, respectively. The Km values for coniferyl acetate and coumaryl acetate were 438.4 ± 44.3 μM and 480.30 ± 86.61 μM, respectively. The catalytic efficiency of IvAIS1 for coniferyl acetate was found to be higher than that for coumaryl acetate. The gene expression profiles showed that IvAIS1 accumulated in the roots, leaves, and fruits, but the levels were relatively low in the stems and flowers of .
This study showed a putative -anol/isoeugenol synthase responsible for converting coumaryl acetate to -anol in It expands our current knowledge of the enzymes involved in -anethole biosynthesis.
[植物名称]是中国南方的一种本土植物，中国是其主要产地。反式茴香脑（-茴香脑）是[植物名称]挥发性芳香成分中的关键成分，具有抗癌和抗糖尿病等治疗作用。然而，其在[植物名称]中的生物合成途径鲜有报道。
在本研究中，我们克隆并在[植物名称]中表达了编码-醇/异丁香酚合酶（IvAIS1）的cDNA。测定了IvAIS1的特性，并通过实时聚合酶链反应测量了其在不同组织中的基因表达。
IvAIS1蛋白与异丁香酚合酶的同一性为76%，这两种蛋白在IGS和EGS进化枝中紧密聚类。IvAIS1表现出依赖NADPH的酶活性和双产物特异性，它分别将乙酸香豆酯和乙酸松柏酯转化为-醇（-茴香脑的前体）和异丁香酚。乙酸松柏酯和乙酸香豆酯的Km值分别为438.4±44.3 μM和480.30±86.61 μM。发现IvAIS1对乙酸松柏酯的催化效率高于对乙酸香豆酯的催化效率。基因表达谱显示，IvAIS1在[植物名称]的根、叶和果实中积累，但在茎和花中的水平相对较低。
本研究表明在[植物名称]中存在一种推定的-醇/异丁香酚合酶，负责将乙酸香豆酯转化为-醇。它扩展了我们目前对参与-茴香脑生物合成的酶的认识。
