Liu Bo, Wang Peng, Lv Xiangyang
Department of Trauma Surgery, Hebei Port Group Co LTD. Qinhuangdao Hospital of Integrated Chinese and Western Medicine, Qinhuangdao, Hebei, 066003, China.
Department of Spine Surgery, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, China.
Open Life Sci. 2025 Mar 21;20(1):20221015. doi: 10.1515/biol-2022-1015. eCollection 2025.
This research investigates the therapeutic efficacy of phytol-mixed micelles in mitigating dexamethasone (Dex)-induced osteoporosis in zebrafish, with a particular focus on scale regeneration. Osteoporosis was induced in zebrafish through exposure to Dex, and the effects of phytol-mixed micelles were evaluated in this model. Following phytol therapy, bone mineralization was assessed using calcium, phosphorus, and alizarin red staining tests. Additionally, commercially available kits quantified the levels of tartrate-resistant acid phosphatase (TRAP), hydroxyproline (HP), and alkaline phosphatase (ALP). The mRNA expression levels of MMP3, osteopontin (OPN), and mitogen-activated protein kinase (MAPK) were examined using reverse transcription polymerase chain reaction (RT-PCR). The findings indicated that phytol significantly increased calcium and phosphorus concentrations. Phytol-mixed micelle therapy led to increased calcium deposition and enhanced bone formation, as evidenced by alizarin red staining. Moreover, phytol administration resulted in increased HP content and upregulated ALP and TRAP activities in zebrafish. RT-PCR tests demonstrated that phytol plays a role in the restoration of the MMP3-OPN-MAPK pathway. In summary, this research highlights the potential of phytol-mixed micelles in ameliorating Dex-induced osteoporosis in zebrafish. Clarifying phytol's mechanism, particularly its stimulation of the MMP3-OPN-MAPK pathway, provides insight into its role in facilitating bone remodeling.
本研究调查了叶绿醇混合胶束在减轻斑马鱼地塞米松（Dex）诱导的骨质疏松症方面的治疗效果，特别关注鳞片再生。通过让斑马鱼接触Dex诱导骨质疏松症，并在此模型中评估叶绿醇混合胶束的效果。叶绿醇治疗后，使用钙、磷和茜素红染色试验评估骨矿化情况。此外，市售试剂盒定量检测了抗酒石酸酸性磷酸酶（TRAP）、羟脯氨酸（HP）和碱性磷酸酶（ALP）的水平。使用逆转录聚合酶链反应（RT-PCR）检测MMP3、骨桥蛋白（OPN）和丝裂原活化蛋白激酶（MAPK）的mRNA表达水平。结果表明，叶绿醇显著提高了钙和磷的浓度。茜素红染色证明，叶绿醇混合胶束治疗导致钙沉积增加和骨形成增强。此外，给予叶绿醇导致斑马鱼中HP含量增加以及ALP和TRAP活性上调。RT-PCR试验表明，叶绿醇在恢复MMP3-OPN-MAPK途径中发挥作用。总之，本研究突出了叶绿醇混合胶束在改善斑马鱼Dex诱导的骨质疏松症方面的潜力。阐明叶绿醇的作用机制，特别是其对MMP3-OPN-MAPK途径的刺激作用，有助于深入了解其在促进骨重塑中的作用。
