Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.
Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.
Biomed Pharmacother. 2018 Jan;97:995-999. doi: 10.1016/j.biopha.2017.11.029. Epub 2017 Nov 8.
The current study was to evaluate the protective effects of Chinese medicine prescription Zuogui Pill (ZGP) on osteoporosis (OP) in zebrafish larvae induced by dexamethasone. Alizarin Red staining, calcium and phosphorus determination were performed to evaluate the effect of ZGP on bone mineralization. Hydroxyproline (HP), Alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits. We found that ZGP had positive effects in increasing bone mineral content (BMC), strengthening bone biomechanical, promoting bone formation, inhibiting bone resorption, and mediating protein levels of TGF-β1/Smads signaling pathway. The findings demonstrated that ZGP treatments inhibited the phosphorylation of TGF-β and p-Smad 3 as well as the expressions of collagen I and collagen II by western blot. Taken together, we demonstrated that ZGP may prevent osteoporosis via reversing the imbalance of bone fomation/bone resorption and activating the TGF-β-Smad signal.
本研究旨在评估中药方剂左归丸（ZGP）对皮质酮诱导的斑马鱼幼鱼骨质疏松症（OP）的保护作用。采用茜素红染色、钙磷测定法评价 ZGP 对骨矿化的作用。采用商业试剂盒测定羟脯氨酸（HP）、碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRAP）。结果发现，ZGP 能增加骨矿物质含量（BMC）、增强骨生物力学、促进骨形成、抑制骨吸收，并调节 TGF-β1/Smads 信号通路的蛋白水平。Western blot 结果表明，ZGP 处理能抑制 TGF-β 的磷酸化和 p-Smad3 的表达，以及胶原 I 和胶原 II 的表达。综上所述，我们证实 ZGP 可能通过逆转骨形成/骨吸收的失衡和激活 TGF-β-Smad 信号来预防骨质疏松症。
