吴茱萸碱对斑马鱼皮质酮诱导骨质疏松的预防作用。
Preventive Effects of Evodiamine on Dexamethasone-Induced Osteoporosis in Zebrafish.
机构信息
The TCM Hospital of Wuxi affiliated, Nanjing University of Chinese Medicine, Department of Traumatology & Orthopedics, China.
Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
出版信息
Biomed Res Int. 2019 Jan 22;2019:5859641. doi: 10.1155/2019/5859641. eCollection 2019.
The aim of this study was to investigate the effect of evodiamine (EV) on dexamethasone-induced osteoporosis in zebrafish. Zebrafish larvae were exposed to different concentrations of dexamethasone to obtain the osteoporosis in zebrafish. Calcium, phosphorus, and alizarin red staining determination were performed to evaluate the effects of EV on bone mineralization. Alkaline phosphatase (ALP), hydroxyproline (HP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits. The expression of MMP3-OPN-MAPK pathway in zebrafish was measured by Western blot. RT-PCR was used to determine mRNA levels of MMP3, OPN, and MAPK. EV could significantly increase the content of calcium and phosphorus. The results of alizarin red staining showed that EV could significantly increase the calcium sink of horse fish, increasing the area of bone formation. EV could increase the content of hydroxyproline in zebrafish. EV also increased ALP and TRAP in zebrafish. Western blot and RT-PCR results showed that EV restored the MMP3-OPN-MAPK pathway in zebrafish. In conclusion, we found that EV can alleviate dexamethasone-induced osteoporosis in zebrafish. The mechanism is related to activating MMP3-OPN-MAPK pathway and then activating bone remodeling.
本研究旨在探讨吴茱萸碱（EV）对斑马鱼皮质酮诱导骨质疏松症的影响。将斑马鱼幼虫暴露于不同浓度的皮质酮中，以获得斑马鱼骨质疏松症模型。通过钙、磷和茜素红染色测定来评估 EV 对骨矿化的影响。通过商业试剂盒还测定碱性磷酸酶（ALP）、羟脯氨酸（HP）和抗酒石酸酸性磷酸酶（TRAP）的含量。通过 Western blot 测定 MMP3-OPN-MAPK 通路在斑马鱼中的表达。通过 RT-PCR 测定 MMP3、OPN 和 MAPK 的 mRNA 水平。EV 可显著增加钙和磷的含量。茜素红染色结果表明，EV 可显著增加马鱼的钙沉淀，增加骨形成面积。EV 可增加斑马鱼羟脯氨酸的含量。EV 还增加了斑马鱼中的 ALP 和 TRAP。Western blot 和 RT-PCR 结果表明，EV 恢复了斑马鱼中的 MMP3-OPN-MAPK 通路。总之，我们发现 EV 可减轻斑马鱼皮质酮诱导的骨质疏松症。其机制与激活 MMP3-OPN-MAPK 通路，进而激活骨重塑有关。
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