Inhibition of carbohydrate digestive enzymes by a complementary essential oil blend: in silico and mixture design approaches.

BACKGROUND

The increasing demand for natural alternatives in diabetes treatment has driven research into plant-derived metabolites, particularly essential oils (EOs) with bioactive properties. This study aims to optimize an EO mixture for inhibiting two key enzymes involved in glucose digestion: pancreatic α-amylase and intestinal α-glucosidase.

METHODS

Essential oils were extracted from three Moroccan medicinal plants: false yellowhead ( L.), rose geranium ( L'Hér.), and lemongrass ( (DC.) Stapf.). Gas chromatography-mass spectrometry (GC-MS) analysis identified key metabolites in each EO. A statistical mixture design was employed to evaluate different EO ratios for their inhibitory effects on α-amylase and α-glucosidase. Additionally, density functional theory (DFT) calculations and molecular docking simulations were conducted to assess the key metabolites' electronic properties and interaction potential with target enzymes.

RESULTS

GC-MS analysis identified 32 metabolites in . , with citronellol (18.67%), eucalyptol (13.30%), and 2-octen-1-ol (8.12%) as major components. . contained 18 metabolites, dominated by 2-camphanol acetate (51.12%) and camphol (19.32%), while had 23 metabolites, with α-citral (24.70%) and 2-isopropenyl-5-methylhex-4-enal (29.25%) as key constituents. The optimal formulation for α-glucosidase inhibition was a binary mixture of 73% and 27% . In contrast, the best blend for α-amylase inhibition consisted of 56% and 44% . DFT calculations confirmed the electrophilic nature of key metabolites, supporting their potential for enzyme interaction. Molecular docking simulations suggested that these phytochemicals could exhibit stronger inhibitory effects than acarbose, a widely used antidiabetic drug.

CONCLUSION

These findings highlight the potential of optimized EO formulations as natural alternatives for managing hyperglycemia and developing novel diabetes therapies.