Taibi Mohamed, Elbouzidi Amine, Haddou Mounir, Baraich Abdellah, Loukili El Hassania, Moubchir Tarik, Allali Aimad, Bellaouchi Reda, Asehraou Abdeslam, Addi Mohamed, Salamatullah Ahmad Mohammad, Bourhia Mohammed, Siddique Farhan, El Guerrouj Bouchra, Chaabane Khalid
Laboratoire d'Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco.
Centre de L'Oriental des Sciences et Technologies de L'Eau et de L'Environnement (COSTEE), Université Mohammed Premier, Oujda, 60000, Morocco.
Heliyon. 2024 Apr 9;10(8):e29459. doi: 10.1016/j.heliyon.2024.e29459. eCollection 2024 Apr 30.
The aim of this study is to explore the pharmacological properties of the essential oil derived from Duby (PVEO), a medicinal plant native to Morocco, focusing on its antidiabetic, anti-tyrosinase, and anti-inflammatory effects. Additionally, the study aims to characterize the phytochemical composition of PVEO and evaluate its potential as a natural therapeutic alternative for various health conditions. To achieve this, phytochemical analysis was conducted using gas chromatography-mass spectrometry (GC-MS). Furthermore, in vitro assessments were conducted to investigate PVEO's antidiabetic activity by inhibiting α-amylase, xanthine oxidase, and α-glucosidase. Tests were also undertaken to evaluate the anti-inflammatory effect of PVEO on RAW 264.7 cells stimulated by lipopolysaccharide (LPS), as well as its efficacy as an anti-tyrosinase agent and its lipoxygenase inhibition activity. The results of the phytochemical analysis revealed that PVEO is rich in terpene compounds, with percentages of 40.35 % γ-terpinene, 22.40 % carvacrol, and 19.77 % β-cymene. Moreover, in vitro evaluations demonstrated that PVEO exhibits significant inhibitory activity against α-amylase, xanthine oxidase, and α-glucosidase, indicating promising antidiabetic, and anti-gout potential. Furthermore, PVEO showed significant anti-tyrosinase activity, with an IC50 of 27.39 ± 0.44 μg/mL, and remarkable lipoxygenase inhibition (87.33 ± 2.6 %), suggesting its candidacy for dermatoprotection. Additionally, PVEO displayed a dose-dependent capacity to attenuate the production of NO and PGE, two inflammatory mediators implicated in various pathologies, without compromising cellular viability. The findings of this study provide a solid foundation for future research on natural therapies and the development of new drugs, highlighting the therapeutic potential of PVEO in the treatment of gout, diabetes, pigmentation disorders, and inflammation.
本研究的目的是探索源自摩洛哥本土药用植物杜比（PVEO）的精油的药理特性，重点关注其抗糖尿病、抗酪氨酸酶和抗炎作用。此外，该研究旨在表征PVEO的植物化学成分，并评估其作为各种健康状况的天然治疗替代品的潜力。为实现这一目标，使用气相色谱 - 质谱联用仪（GC - MS）进行了植物化学分析。此外，进行了体外评估，通过抑制α - 淀粉酶、黄嘌呤氧化酶和α - 葡萄糖苷酶来研究PVEO的抗糖尿病活性。还进行了测试，以评估PVEO对脂多糖（LPS）刺激的RAW 264.7细胞的抗炎作用，以及其作为抗酪氨酸酶剂的功效及其脂氧合酶抑制活性。植物化学分析结果表明，PVEO富含萜类化合物，其中γ - 萜品烯占40.35%，香芹酚占22.40%，β - 伞花烃占19.77%。此外，体外评估表明，PVEO对α - 淀粉酶、黄嘌呤氧化酶和α - 葡萄糖苷酶具有显著的抑制活性，显示出有前景的抗糖尿病和抗痛风潜力。此外，PVEO表现出显著的抗酪氨酸酶活性，IC50为27.39±0.44μg/mL，并且具有显著的脂氧合酶抑制作用（87.33±2.6%），表明其具有皮肤保护的潜力。此外，PVEO显示出剂量依赖性能力，可减弱一氧化氮（NO）和前列腺素E（PGE）的产生，这两种炎症介质与多种病理状况有关，且不影响细胞活力。本研究结果为未来天然疗法研究和新药开发提供了坚实基础，突出了PVEO在治疗痛风、糖尿病、色素沉着紊乱和炎症方面的治疗潜力。
