Elbouzidi Amine, Taibi Mohamed, Laaraj Salah, Loukili El Hassania, Haddou Mounir, El Hachlafi Naoufal, Naceiri Mrabti Hanae, Baraich Abdellah, Bellaouchi Reda, Asehraou Abdeslam, Bourhia Mohammed, Nafidi Hiba-Allah, Bin Jardan Yousef A, Chaabane Khalid, Addi Mohamed
Laboratoire d'Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, Morocco.
Euro-Mediterranean University of Fes (UEMF), Fes, Morocco.
Front Chem. 2024 Feb 15;12:1334028. doi: 10.3389/fchem.2024.1334028. eCollection 2024.
: L., also known as Grey-leaved rockrose and locally addressed as šṭab or tûzzâla lbîḍa, is a plant species with a well-established reputation for its health-promoting properties and traditional use for the treatment of various diseases. This research delves into exploring the essential oil extracted from the aerial components of (referred to as CAEO), aiming to comprehend its properties concerning antioxidation, anti-inflammation, antimicrobial efficacy, and cytotoxicity. Firstly, a comprehensive analysis of CAEO's chemical composition was performed through Gas Chromatography-Mass Spectrometry (GC-MS). Subsequently, four complementary assays were conducted to assess its antioxidant potential, including DPPH scavenging, β-carotene bleaching, ABTS scavenging, and total antioxidant capacity assays. The investigation delved into the anti-inflammatory properties via the 5-lipoxygenase assay and the antimicrobial effects of CAEO against various bacterial and fungal strains. Additionally, the research investigated the cytotoxic effects of CAEO on two human breast cancer subtypes, namely, MCF-7 and MDA-MB-231. Chemical analysis revealed camphene as the major compound, comprising 39.21% of the composition, followed by α-pinene (19.01%), bornyl acetate (18.32%), tricyclene (6.86%), and melonal (5.44%). Notably, CAEO exhibited robust antioxidant activity, as demonstrated by the low IC values in DPPH (153.92 ± 4.30 μg/mL) and β-carotene (95.25 ± 3.75 μg/mL) assays, indicating its ability to counteract oxidative damage. The ABTS assay and the total antioxidant capacity assay also confirmed the potent antioxidant potential with IC values of 120.51 ± 3.33 TE μmol/mL and 458.25 ± 3.67 µg AAE/mg, respectively. In terms of anti-inflammatory activity, CAEO displayed a substantial lipoxygenase inhibition at 0.5 mg/mL. Its antimicrobial properties were broad-spectrum, although some resistance was observed in the case of and . CAEO exhibited significant dose-dependent inhibitory effects on tumor cell lines . Additionally, computational analyses were carried out to appraise the physicochemical characteristics, drug-likeness, and pharmacokinetic properties of CAEO's constituent molecules, while the toxicity was assessed using the Protox II web server.
L.，也被称为灰叶岩蔷薇，在当地被称为šṭab或tûzzâla lbîḍa，是一种因其促进健康的特性和用于治疗各种疾病的传统用途而享有盛誉的植物物种。本研究深入探索从L.地上部分提取的精油（称为CAEO），旨在了解其抗氧化、抗炎、抗菌功效和细胞毒性方面的特性。首先，通过气相色谱 - 质谱联用（GC - MS）对CAEO的化学成分进行了全面分析。随后，进行了四项补充试验以评估其抗氧化潜力，包括DPPH清除、β - 胡萝卜素漂白、ABTS清除和总抗氧化能力试验。通过5 - 脂氧合酶试验深入研究其抗炎特性以及CAEO对各种细菌和真菌菌株的抗菌作用。此外，该研究调查了CAEO对两种人类乳腺癌亚型MCF - 7和MDA - MB - 231的细胞毒性作用。化学分析表明莰烯是主要化合物，占成分的39.21%，其次是α - 蒎烯（19.01%）、乙酸龙脑酯（18.32%）、三环烯（6.86%）和甜瓜醛（5.44%）。值得注意的是，CAEO表现出强大的抗氧化活性，如在DPPH（153.92±4.30μg/mL）和β - 胡萝卜素（95.25±3.75μg/mL）试验中低IC值所示，表明其具有对抗氧化损伤的能力。ABTS试验和总抗氧化能力试验也证实了其强大的抗氧化潜力，IC值分别为120.51±3.33 TE μmol/mL和458.25±3.67μg AAE/mg。在抗炎活性方面，CAEO在0.5mg/mL时表现出显著的脂氧合酶抑制作用。其抗菌特性具有广谱性，尽管在[具体菌株1]和[具体菌株2]的情况下观察到一些抗性。CAEO对肿瘤细胞系表现出显著的剂量依赖性抑制作用。此外，进行了计算分析以评估CAEO组成分子的物理化学特性、药物相似性和药代动力学特性，同时使用Protox II网络服务器评估毒性。
