Xiong Shijie, Wan Fan, Jiang Jiajia, Liu Yanfang, Hang Yiqiong, Xue Huiqin, Lu Yang, Su Yong
Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Front Immunol. 2025 Mar 10;16:1530098. doi: 10.3389/fimmu.2025.1530098. eCollection 2025.
extract (CME) and cordycepin (CPN) are biomolecules with a wide range of biological activities, including anti-inflammatory, antioxidant and anti-tumour effects. The research objective wasto investigate the influences of CME and cordycepin CPN on colonic morphology, microbiota composition and colonic metabolomics in lipopolysaccharide (LPS)-challenged piglets.
Twenty-four weaned castrated piglets were randomly divided into four groups: control group (fed basal diets), LPS group (fed basal diets), CPN-LPS group (basal diets + 60 mg/kg cordycepin), and CME-LPS group (basal diets + 60 mg/kg sextract). On the 21st day, the LPS, CPN-LPS, and CME-LPS groups received an injection of 100 μg/kg BW LPS, while the control group was given sterile saline.
The findings demonstrated that CPN or CME attenuated intestinal morphology damage with LPS-challenged piglets. CPN and CME alleviated intestinal microbiota dysbiosis and metabolic disorders under LPS-challenged by enriching serum protein levels, regulating of inflammatory cytokine secretion and altering colonic microbial composition. Colonic microbiota analysis that the CPN improved the relative abundance of Acidobacteriota and inhibited , CME promoted the relative abundance of and group. Meanwhile, the alleviation of colonic damage is achieved through modulation of metabolic pathways linked to tryptophan metabolism, biosynthesis of amino acids and butanoate metabolism.
Conclusively, our preliminary findings reveal that CPN or CME could serve as a beneficial dietary supplement to alleviate gut diseases in weaning piglets.
提取物（CME）和虫草素（CPN）是具有广泛生物活性的生物分子，包括抗炎、抗氧化和抗肿瘤作用。本研究的目的是探讨CME和虫草素CPN对脂多糖（LPS）攻击的仔猪结肠形态、微生物群组成和结肠代谢组学的影响。
24只断奶去势仔猪随机分为四组：对照组（饲喂基础日粮）、LPS组（饲喂基础日粮）、CPN-LPS组（基础日粮+60mg/kg虫草素）和CME-LPS组（基础日粮+60mg/kg提取物）。在第21天，LPS组、CPN-LPS组和CME-LPS组注射100μg/kg体重的LPS，而对照组注射无菌生理盐水。
研究结果表明，CPN或CME减轻了LPS攻击仔猪的肠道形态损伤。CPN和CME通过提高血清蛋白水平、调节炎症细胞因子分泌和改变结肠微生物组成，缓解了LPS攻击下的肠道微生物群失调和代谢紊乱。结肠微生物群分析表明，CPN提高了酸杆菌门的相对丰度并抑制了……，CME促进了……和……组的相对丰度。同时，通过调节与色氨酸代谢、氨基酸生物合成和丁酸代谢相关的代谢途径，减轻了结肠损伤。
总之，我们的初步研究结果表明，CPN或CME可以作为一种有益的膳食补充剂，以减轻断奶仔猪的肠道疾病。
