Pan Zhenzhi, Zhu Yanxiang, Lin Chaoyang, Tang Mengzhen, Shen Zhicheng, Zheng Ting
Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Hainan Research Institute, Zhejiang University, Sanya, 572025, China.
Plant Cell Rep. 2025 Mar 25;44(4):87. doi: 10.1007/s00299-025-03463-4.
Transgenic soybean event CAL-16 expressing fusion Bt protein Cry1Ab-Vip3A was developed for conferring broad-spectrum resistance to lepidopteran pests Lepidopteran insect species are important soybean pests causing significant yield loss and quality degradation worldwide. Transgenic soybeans expressing crystal (Cry) insecticidal proteins have been utilized for insect pest management. However, the efficacy of currently adopted insect-resistant soybean is challenged by insect resistance evolution. Vegetative insecticidal proteins (Vips) are highly active against a broad spectrum of lepidopteran insects. They differ from Cry in modes of action, and show great potential for lepidopteran pest management. Here, we report the creation and characterization of a transgenic soybean event CAL-16 which expresses a fusion protein of Cry1Ab and Vip3A. CAL-16 is a single copy T-DNA insertion transgenic event highly resistant to a broad-spectrum of lepidopteran insects. Insect bioassays demonstrated that CAL-16 caused 100% mortality to neonates of Helicoverpa armigera, Spodoptera litura, Agrotis ipsilon, Spodoptera exigua and Spodoptera frugiperda. Field trial also demonstrated its excellent resistance to Leguminivora glycinivorella, a severe pest feeding on soybean seeds. The expression of the fusion protein was found to be constitutively high in CAL-16 throughout developmental stages, and highly stable over 12 generations. Moreover, there was no statistical difference in agronomic traits between CAL-16 and its non-transgenic recipient control plants in field trial. In conclusion, CAL-16 is an elite soybean event with high efficacy toward major lepidopteran pests. It is expected to be released for commercial cultivation in the near future in China as it has been deregulated in China in 2023.
表达融合Bt蛋白Cry1Ab-Vip3A的转基因大豆事件CAL-16被培育出来，用于赋予对鳞翅目害虫的广谱抗性。鳞翅目昆虫是重要的大豆害虫，在全球范围内造成严重的产量损失和品质下降。表达晶体（Cry）杀虫蛋白的转基因大豆已被用于害虫管理。然而，目前采用的抗虫大豆的功效受到害虫抗性进化的挑战。营养期杀虫蛋白（Vip）对广谱鳞翅目昆虫具有高活性。它们在作用方式上与Cry不同，在鳞翅目害虫管理方面显示出巨大潜力。在此，我们报道了表达Cry1Ab和Vip3A融合蛋白的转基因大豆事件CAL-16的创制和特性分析。CAL-16是一个单拷贝T-DNA插入的转基因事件，对广谱鳞翅目昆虫具有高度抗性。昆虫生物测定表明，CAL-16对棉铃虫、斜纹夜蛾、小地老虎、甜菜夜蛾和草地贪夜蛾的幼虫造成100%的死亡率。田间试验也证明了它对豆蚀叶野螟（一种严重取食大豆种子的害虫）具有优异的抗性。发现融合蛋白在CAL-16的整个发育阶段组成型高表达，并且在12代中高度稳定。此外，在田间试验中，CAL-16与其非转基因受体对照植株在农艺性状上没有统计学差异。总之，CAL-16是一个对主要鳞翅目害虫具有高效性的优良大豆事件。由于它在2023年已在中国解除管制，预计在不久的将来将在中国被批准用于商业种植。
