Pan Jialu, Meng Qi, Zhang Li, Zhang Caidan, Wu Shaohua, Zhai Huiyuan
College of Textiles & Clothing, Qingdao University, Qingdao 266071, China.
Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Colloids Surf B Biointerfaces. 2025 Aug;252:114648. doi: 10.1016/j.colsurfb.2025.114648. Epub 2025 Mar 22.
Design and construction of novel dressings with appropriate structure and multiple bio-functions are urgently required for the wound treatment applications. In this study, a series of wound dressings composed of amine-modified polysuccinimide (PSI)/polycaprolactone (PCL) hydrogel nanofibers were fabricated by using the conjugated electrospinning and the chemical crosslinking post-treatment. All the dressings with different PSI/PCL ratios could effectively maintain the fibrous morphology even after the chemical crosslinking process. The ultimate stress and Young's modulus of the PSI-NH/PCL dressings significantly increased with higher PCL content. In particular, when the proportion of PCL reached 40 % (with a PSI/PCL mass ratio of 60/40), the PSI-NH/PCL dressing exhibited the most excellent mechanical properties, with a Young's modulus of 31.9 ± 8.9 MPa and an ultimate stress of 7.4 ± 0.4 MPa, which were significantly higher than the other samples. Furthermore, all samples exhibited a high swelling ratio of over 350 %, although a noticeable decrease in swelling properties was observed when the content of PCL increased. In addition, all the PSI-NH/PCL dressings showed a high antibacterial activity (> 99 %) against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), which demonstrated that the addition of PCL has no significant influence on the antibacterial activity of the PSI. Moreover, all the PSI-NH/PCL nanofiber hydrogel dressings had excellent biocompatibility to human dermal fibroblasts (HDFs). Importantly, all the PSI-NH/PCL nanofiber hydrogel dressings presented excellent hemostatic capacity. Notably, even the dressing with the highest PCL content still demonstrated a 71.8 % reduction in bleeding compared to the control group. In summary, our PSI-NH/PCL nanofiber hydrogel dressings with high absorption, biocompatibility and extracellular matrix (ECM)-mimicking capacities, as well as great mechanical, antibacterial, and hemostatic properties are excellent as a promising wound dressing.
伤口治疗应用迫切需要设计和构建具有适当结构和多种生物功能的新型敷料。在本研究中，通过共轭静电纺丝和化学交联后处理制备了一系列由胺改性聚琥珀酰亚胺（PSI）/聚己内酯（PCL）水凝胶纳米纤维组成的伤口敷料。即使经过化学交联过程，所有不同PSI/PCL比例的敷料仍能有效保持纤维形态。随着PCL含量的增加，PSI-NH/PCL敷料的极限应力和杨氏模量显著提高。特别是，当PCL比例达到40%（PSI/PCL质量比为60/40）时，PSI-NH/PCL敷料表现出最优异的机械性能，杨氏模量为31.9±8.9MPa，极限应力为7.4±0.4MPa，显著高于其他样品。此外，所有样品均表现出超过350%的高溶胀率，尽管随着PCL含量的增加，溶胀性能明显下降。此外，所有PSI-NH/PCL敷料对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）均表现出高抗菌活性（>99%），这表明PCL的添加对PSI的抗菌活性没有显著影响。此外，所有PSI-NH/PCL纳米纤维水凝胶敷料对人皮肤成纤维细胞（HDFs）具有优异的生物相容性。重要的是，所有PSI-NH/PCL纳米纤维水凝胶敷料均具有优异的止血能力。值得注意的是，即使PCL含量最高的敷料与对照组相比，出血仍减少了71.8%。综上所述，我们的PSI-NH/PCL纳米纤维水凝胶敷料具有高吸收性、生物相容性和细胞外基质（ECM）模拟能力，以及出色的机械、抗菌和止血性能，是一种很有前景的伤口敷料。
