Huang Ying, Wang Zhengao, Fu Meimei, Lin Dongsong, Zhao Fuyu, Wu Keke, Ren Xia, Fu Xianjun, Zhou Wuyi
Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine, Qingdao Key Laboratory of Research in Marine Traditional Chinese Medicine, Qingdao Key Technology Innovation Center of Marine Traditional Chinese Medicines Deep Development and Industrialization, Qingdao 266114, China; Research Institute for Marine Traditional Chinese Medicine, The SATCM's Key Unit of Discovering and Developing New Marine TCM Drugs, Key Laboratory of Marine Traditional Chinese Medicine in Shandong Universities, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
College of Materials and Energy Key, Laboratory for Biobased Materials and Energy of Ministry of Education, Research Center of Biomass 3D Printing Materials, South China Agricultural University, Guangzhou 510642, China.
Colloids Surf B Biointerfaces. 2025 Aug;252:114703. doi: 10.1016/j.colsurfb.2025.114703. Epub 2025 Apr 12.
Effective wound management remains a major clinical challenge, especially in preventing infections and promoting rapid healing. However, traditional wound dressings often lack multifunctionality, limiting their ability to support hemostasis and antibacterial protection. To address this challenge, in this work, a novel wound dressing was developed utilizing nano-cuttlebone particles, which are rich in calcium carbonate and chitin, to enhance hemostatic and antimicrobial functionality. The dressing, composed of polyvinyl alcohol, sodium alginate, and nano-cuttlebone, addresses the need for advanced wound care solutions. The particle size distribution of the powder was evaluated using a laser particle size analyzer, and the particle size of the nano-cuttlebone was around 75 nm. The drug release experiment showed that the drug release of nano cuttlebone hydrogel was 1.4 times that of micro cuttlebone hydrogel in 48 h. The antibacterial activity of the nano-cuttlebone-doped dressing against S. aureus was 97.32 % and against E. coli was 99.99 %. The cell scratch test showed that the nano-cuttlebone hydrogel gradually migrated to the central area, and its migration rate was 61.43 % after 12 hours. The hydrogel also demonstrated excellent hemostatic ability in a mouse liver injury model and could rapidly stop bleeding within 1 min. In a full-thickness wound model, the nano-cuttlebone hydrogel enhanced collagen deposition and promoted faster wound closure. This study highlights the potential of marine biomaterials for developing multifunctional wound dressings to improve patient outcomes.
有效的伤口管理仍然是一项重大的临床挑战，尤其是在预防感染和促进快速愈合方面。然而，传统的伤口敷料往往缺乏多功能性，限制了它们支持止血和抗菌保护的能力。为了应对这一挑战，在这项工作中，利用富含碳酸钙和几丁质的纳米乌贼骨颗粒开发了一种新型伤口敷料，以增强止血和抗菌功能。该敷料由聚乙烯醇、海藻酸钠和纳米乌贼骨组成，满足了对先进伤口护理解决方案的需求。使用激光粒度分析仪评估了粉末的粒度分布，纳米乌贼骨的粒度约为75纳米。药物释放实验表明，纳米乌贼骨水凝胶在48小时内的药物释放量是微乌贼骨水凝胶的1.4倍。掺杂纳米乌贼骨的敷料对金黄色葡萄球菌的抗菌活性为97.32%，对大肠杆菌的抗菌活性为99.99%。细胞划痕试验表明，纳米乌贼骨水凝胶逐渐迁移至中心区域，12小时后其迁移率为61.43%。该水凝胶在小鼠肝损伤模型中也表现出优异的止血能力，可在1分钟内迅速止血。在全层伤口模型中，纳米乌贼骨水凝胶增强了胶原蛋白沉积并促进伤口更快愈合。这项研究突出了海洋生物材料在开发多功能伤口敷料以改善患者预后方面的潜力。
