Huang Xing-Hua, Huang Yi-Yao, Gao Wu, Zhang Yi-da, Liu Xiao-Yan, Zhang Hai-Xia
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Se Pu. 2025 Apr 8;43(4):388-393. doi: 10.3724/SP.J.1123.2024.06010.
Atrazine is a triazine pesticide that interferes with normal physiological activities, induces oxidative stress, and is significantly toxic to plants. Therefore, the ability to quantitatively detect atrazine and study the mechanisms responsible for its toxicity are crucial for ensuring food safety and maintaining plant growth. Herein, a method was developed by QuEChERS combined with fluorescence derivatization to rapidly, sensitively, and quantitatively detect atrazine in real samples. This approach offers excellent selectivity and high sensitivity, and is simple to operate. In addition, we also investigated the effect of atrazine on catalase activity through enzyme-activity assays and molecular-docking studies to explore the mechanism responsible for the toxicity of atrazine on the molecular level. This experiment involves knowledge related to sample pretreatment, spectroscopy, and molecular docking, while the instruments used are widely available in colleges and universities and are very suitable for teaching open experiments. This experiment aims to provide students with a comprehensive understanding of experimental operations integrated with theory, as well as applied research aimed at helping students understand the use of separation materials, the selection and application of fluorescent derivatization reagents, the principles of analytical instruments, the use of enzymes, and the principles of molecular docking. This experiment is expected to enhance students' awareness of people's livelihoods, analytical chemistry concepts, and operational standards, which will guide the development of their scientific research thinking and improve their abilities to solve real-world problems.
莠去津是一种三嗪类农药，会干扰正常生理活动，引发氧化应激，对植物具有显著毒性。因此，定量检测莠去津的能力以及研究其毒性机制对于确保食品安全和维持植物生长至关重要。在此，开发了一种将QuEChERS与荧光衍生化相结合的方法，用于快速、灵敏且定量地检测实际样品中的莠去津。该方法具有出色的选择性和高灵敏度，且操作简便。此外，我们还通过酶活性测定和分子对接研究，研究了莠去津对过氧化氢酶活性的影响，以从分子水平探索莠去津毒性的作用机制。本实验涉及样品预处理、光谱学和分子对接等相关知识，所使用的仪器在高校中广泛可得，非常适合用于教学开放实验。本实验旨在让学生全面了解与理论相结合的实验操作，以及旨在帮助学生理解分离材料的使用、荧光衍生化试剂的选择与应用、分析仪器的原理、酶的使用以及分子对接原理的应用研究。本实验有望增强学生的民生意识、分析化学概念和操作规范，这将引导他们科研思维的发展，提高他们解决实际问题的能力。
