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获能对公牛精子蛋白质组图谱和线粒体参数的影响

Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.

作者信息

Castelló-Ruiz María, Gacem Sabrina, Sánchez Del Pino Manuel M, Hidalgo Carlos O, Tamargo Carolina, Álvarez-Rodríguez Manuel, Yániz Jesús L, Silvestre Miguel A

机构信息

Department of Cellular Biology, Functional Biology and Physical Anthropology, Universitat de València, Burjassot 46100, Spain.

Unidad Mixta de Investigación Cerebrovascular, Instituto de Investigación Sanitaria La Fe, Hospital Universitario y Politécnico La Fe, Valencia 46026, Spain.

出版信息

J Proteome Res. 2025 Apr 4;24(4):1817-1831. doi: 10.1021/acs.jproteome.4c00910. Epub 2025 Mar 25.

DOI:10.1021/acs.jproteome.4c00910
PMID:40133237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12128097/
Abstract

Sperm capacitation is a critical process for fertilization. This work aims to analyze the effect capacitation had on the proteome and mitochondrial parameters of bull spermatozoa. Viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS) were assessed by flow cytometry in noncapacitated (NC) and capacitated (IVC) sperm. Proteome was evaluated using SWATH-MS. capacitation significantly induced a decrease in sperm viability, a high MMP, and an increase in mROS production. Within the group of living spermatozoa, the capacitation significantly induced a decrease in healthy mitochondrial spermatozoa, as well as an increase in mROS production, without affecting the MMP intensity. A total number of 72 differentially abundant proteins were found of which 63 were over-represented in the NC sperm group and 9 in the IVC sperm group. It was observed that many proteins associated with the sperm membrane and acrosome were lost during the capacitation process. For the IVC sperm, the functional enrichment was found in proteins related to the oxidative phosphorylation process. Our results indicate that the capacitation process induces a significant loss of seminal plasma-derived membrane proteins and a significant increase in proteins related with the oxidative phosphorylation (OXPHOS) pathway. Data are available via ProteomeXchange with identifiers PXD056424 and PXD042286.

摘要

精子获能是受精的关键过程。本研究旨在分析获能对公牛精子蛋白质组和线粒体参数的影响。通过流式细胞术评估未获能（NC）和获能（IVC）精子的活力、线粒体膜电位（MMP）和活性氧（mROS）。使用SWATH-MS评估蛋白质组。获能显著导致精子活力下降、MMP升高以及mROS产生增加。在活精子组中，获能显著导致健康线粒体精子减少以及mROS产生增加，而不影响MMP强度。共发现72种差异丰富的蛋白质，其中63种在NC精子组中过度表达，9种在IVC精子组中过度表达。观察到许多与精子膜和顶体相关的蛋白质在获能过程中丢失。对于IVC精子，在与氧化磷酸化过程相关的蛋白质中发现了功能富集。我们的结果表明，获能过程导致精浆来源的膜蛋白显著丢失，以及与氧化磷酸化（OXPHOS）途径相关的蛋白质显著增加。数据可通过ProteomeXchange获得，标识符为PXD056424和PXD042286。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/690da59da3bf/pr4c00910_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/80ddf262751f/pr4c00910_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/1f83bd6dc60f/pr4c00910_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/698f56416b2d/pr4c00910_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/f1fbed191660/pr4c00910_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/c66d2cc930d3/pr4c00910_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/fd8dc309e75e/pr4c00910_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/690da59da3bf/pr4c00910_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/80ddf262751f/pr4c00910_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/1f83bd6dc60f/pr4c00910_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/698f56416b2d/pr4c00910_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/f1fbed191660/pr4c00910_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/c66d2cc930d3/pr4c00910_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/fd8dc309e75e/pr4c00910_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f12f/12128097/690da59da3bf/pr4c00910_0007.jpg

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Anim Reprod Sci. 2024 Apr;263:107441. doi: 10.1016/j.anireprosci.2024.107441. Epub 2024 Feb 21.
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Cryopreservation of highly extended pig spermatozoa remodels its proteome and counteracts polyspermic fertilization in vitro.高度延伸的猪精子的冷冻保存重塑了其蛋白质组，并在体外阻止了多精受精。
Andrology. 2024 Sep;12(6):1356-1372. doi: 10.1111/andr.13575. Epub 2023 Dec 22.
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