Yang Jie, Zhang Donghui, Jiang Wei
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.
Department of Biological Repositories, Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430071, China.
Cell Regen. 2025 Mar 26;14(1):11. doi: 10.1186/s13619-025-00230-4.
Accumulated studies have demonstrated that long noncoding RNAs (lncRNAs) play crucial regulatory roles in diverse biological processes, such as embryonic development and cell differentiation. Comprehensive transcriptome analysis identifies extensive lncRNAs, gradually elucidating their functions across various contexts. Recent studies have highlighted the essential role of lncRNAs in definitive endoderm differentiation, underscoring their importance in early development. In this review, we have analyzed the features of overlapping, proximal, and desert lncRNAs, classified by genomic location, in pluripotent stem cells (PSCs) and the differentiation derivatives. Furthermore, we focus on the endoderm lineage and review the latest advancements in lncRNA identification and their distinct regulatory mechanisms. By consolidating current knowledge, we aim to provide a clearer perspective on how lncRNAs contribute to endoderm differentiation in different manners.
大量研究表明，长链非编码RNA（lncRNA）在多种生物学过程中发挥着关键的调控作用，如胚胎发育和细胞分化。全面的转录组分析鉴定出了大量的lncRNA，逐渐阐明了它们在各种情况下的功能。最近的研究强调了lncRNA在确定内胚层分化中的重要作用，突显了它们在早期发育中的重要性。在这篇综述中，我们分析了根据基因组位置分类的重叠lncRNA、近端lncRNA和荒漠lncRNA在多能干细胞（PSC）及其分化衍生物中的特征。此外，我们聚焦于内胚层谱系，回顾lncRNA鉴定的最新进展及其独特的调控机制。通过整合当前的知识，我们旨在更清晰地了解lncRNA如何以不同方式促进内胚层分化。
