Zheng Lei, Zhao Biao, Ji Run, Zhang Zhenxi, Liu Yutong, Zhao Xiaoqi, Cai Jing, Qiao Tong
Department of Vascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Department of Vascular Surgery and Intervention, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
Front Pharmacol. 2025 Mar 11;16:1509860. doi: 10.3389/fphar.2025.1509860. eCollection 2025.
This research aims to explore whether Berbamine (BBM) can mitigate tissue damage in mice resulting from hind limb muscle ischemia-reperfusion by scavenging lipid ROS and inhibiting p65 nuclear translocation. The hind limb ischemia-reperfusion (IR) injury model in mice was employed. Forty-eight mice (n = 12 per group) were randomly allocated into four groups: Sham group, IR group, IR + BBM (20 mg/kg) group, and IR + BBM (50 mg/kg) group. We observed that BBM pretreatment shielded against muscle damage and diminished levels of cell apoptosis compared to the control group. The mechanism likely involves reducing the movement of p65 into the nucleus and lessening the build-up of lipid ROS in muscle tissue. This action helps to decrease the release of substances that cause inflammation, ultimately reducing the inflammation in tissues that occurs as a result of hind limb IR. Our findings suggest that BBM has a protective impact on hindlimb ischemia-reperfusion injury, potentially due to its capacity to eliminate tissue lipid ROS and prevent p65 nuclear translocation.
本研究旨在探讨小檗胺（BBM）是否能通过清除脂质活性氧（ROS）和抑制p65核转位来减轻小鼠后肢肌肉缺血再灌注所致的组织损伤。采用小鼠后肢缺血再灌注（IR）损伤模型。48只小鼠（每组n = 12只）随机分为四组：假手术组、IR组、IR + BBM（20 mg/kg）组和IR + BBM（50 mg/kg）组。我们观察到，与对照组相比，BBM预处理可减轻肌肉损伤并降低细胞凋亡水平。其机制可能涉及减少p65进入细胞核的移动以及减少肌肉组织中脂质ROS的积累。这一作用有助于减少引起炎症的物质的释放，最终减轻后肢IR所致的组织炎症。我们的研究结果表明，BBM对后肢缺血再灌注损伤具有保护作用，这可能归因于其清除组织脂质ROS和防止p65核转位的能力。
