Morganti Dario, Longo Valeria, Leonardi Antonio Alessio, Irrera Alessia, Colombo Paolo, Fazio Barbara
CNR IMM-ME, Institute for Microelectronics and Microsystems, Viale F.S. d'Alcontres 31, I-98166 Messina, Italy.
CNR DSFTM, Department of Physical Sciences and Technologies of Matter, Piazzale Aldo Moro, 7, I-00185 Roma, Italy.
Biosensors (Basel). 2025 Mar 13;15(3):182. doi: 10.3390/bios15030182.
Accurate identification and characterization of allergenic proteins at the molecular level are essential for pinpointing the specific protein structures responsible for allergic reactions, thus advancing the development of precise diagnostic tests. Significant efforts have been focused on novel experimental techniques aimed at deepening the understanding of the underlying molecular mechanisms of these reactions. In this work, we show, for the first time to our knowledge, the unique Raman fingerprint of three (Par j) allergenic proteins. These proteins are typically present in pollen and are known to trigger severe respiratory diseases. In our research, we further exploited the surface-enhanced Raman scattering (SERS) effect from an Ag dendrite substrate. This approach provided better discrimination and a comprehensive analysis of the proteins Par j 1, 2, and 4 in hydration conditions, enabling rapid differentiation between them through a spectroscopic study.
在分子水平上准确鉴定和表征过敏原蛋白对于确定引发过敏反应的特定蛋白质结构至关重要，从而推动精确诊断测试的发展。大量努力集中在旨在加深对这些反应潜在分子机制理解的新型实验技术上。在这项工作中，据我们所知，我们首次展示了三种（Par j）过敏原蛋白的独特拉曼指纹。这些蛋白通常存在于花粉中，已知会引发严重的呼吸道疾病。在我们的研究中，我们进一步利用了银树枝状基底的表面增强拉曼散射（SERS）效应。这种方法在水合条件下对Par j 1、2和4蛋白提供了更好的区分和全面分析，通过光谱研究能够快速区分它们。
