Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Henan, Zhengzhou, 450002, People's Republic of China.
Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Henan, Zhengzhou, 450002, People's Republic of China.
Environ Res. 2024 Dec 15;263(Pt 2):120147. doi: 10.1016/j.envres.2024.120147. Epub 2024 Oct 13.
High-temperature stress (HS) severely threatens agricultural production. Pleurotus ostreatus is cultivated in many parts of the world, and its growth is strongly affected by HS. We previously reported that metabolic rearrangement occurred in HS, but the gene expression levels of several key enzymes remained unchanged. Therefore, in this study, we investigated the contribution of posttranslational modifications of proteins to HS resistance in P. ostreatus. We found that the level of acetylation of P. ostreatus decreased under short-term HS treatment and increased as the duration of HS treatment increased. Acetylation omics revealed that almost all metabolic enzymes were acetylated. We found that deacetylation under HS can improve the growth recovery ability of mycelia, the activity of matrix-degrading enzyme, and the contents of antioxidants, such as nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione (GSH), but can decreased HO levels. In vitro acetylation experiments and point mutations revealed that the deacetylase SIRT2 increased the activity of glutathione transferases (GSTs) by deacetylating GST1 66K, GST2 206K, and GST2 233K. Together, SIRT2 is activated by short-term HS and improves its antioxidant activity by deacetylating GSTs, thereby improving the resistance of P. ostreatus to HS. In this study, we identified new non-histone substrate proteins and new lysine acetylation sites of SIRT2 under HS. We also discovered the role of non-histone acetylation in the adaptation of organisms to HS.
高温胁迫（HS）严重威胁农业生产。糙皮侧耳在世界许多地方都有栽培，其生长受到 HS 的强烈影响。我们之前报道过 HS 会导致代谢重排，但几种关键酶的基因表达水平保持不变。因此，在这项研究中，我们研究了蛋白质翻译后修饰对糙皮侧耳抗 HS 的贡献。我们发现，糙皮侧耳的乙酰化水平在短期 HS 处理下降低，随着 HS 处理时间的延长而增加。乙酰化组学表明，几乎所有的代谢酶都被乙酰化。我们发现 HS 下的去乙酰化可以提高菌丝的生长恢复能力、基质降解酶的活性以及烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和谷胱甘肽（GSH）等抗氧化剂的含量，但会降低 HO 水平。体外乙酰化实验和点突变表明，去乙酰酶 SIRT2 通过去乙酰化 GST1 66K、GST2 206K 和 GST2 233K 来增加谷胱甘肽转移酶（GSTs）的活性。总的来说，SIRT2 被短期 HS 激活，并通过去乙酰化 GSTs 提高其抗氧化活性，从而提高糙皮侧耳对 HS 的抗性。在这项研究中，我们鉴定了 HS 下 SIRT2 的新非组蛋白底物蛋白和新赖氨酸乙酰化位点。我们还发现了非组蛋白乙酰化在生物体适应 HS 中的作用。
