Badfar Narjes, Jafarpour Ali, Casanova Federico, Sales Queiroz Lucas, Tilahun Getachew Adane, Jacobsen Charlotte, Jessen Flemming, Gringer Nina
Research Group for Bioactives-Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, Denmark.
Research Group for Food Production Engineering, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, Denmark.
Mar Drugs. 2025 Mar 11;23(3):122. doi: 10.3390/md23030122.
This study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP). ALC-treated PC achieved the highest protein recovery (63.49%), extraction yield (24.73%), and hydrolysis degree (18.10%) ( < 0.05). SFE-treated hydrolysates showed higher zeta potential (-47.23 to -49.93 mV) than non-SFE samples (-25.15 to -38.62 mV) but had larger droplet sizes, indicating lower emulsion stability. SC-ALC displayed reduced fluorescence intensity and a red shift in maximum wavelength. TRYP hydrolysates reduced interfacial tension (20 mN/m), similar to sodium caseinate (Na-Cas, 13 mN/m), but with lesser effects. Dilatational rheology showed TRYP hydrolysates formed stronger, solid-like structures. These results emphasize protease efficacy over SFE for extracting functional compounds, enhancing shrimp waste valorization.
本研究探讨了超临界流体萃取（SFE）与酶水解相结合如何影响从压滤虾废料中回收的肽的结构和功能。通过SFE对冻干的滤饼（PC）进行脱脂，并使用碱性蛋白酶（ALC）和胰蛋白酶（TRYP）进行水解。经ALC处理的PC实现了最高的蛋白质回收率（63.49%）、提取率（24.73%）和水解度（18.10%）（<0.05）。经SFE处理的水解产物显示出比未进行SFE处理的样品（-25.15至-38.62 mV）更高的ζ电位（-47.23至-49.93 mV），但液滴尺寸更大，表明乳液稳定性较低。超临界流体萃取-碱性蛋白酶（SC-ALC）显示出荧光强度降低且最大波长发生红移。TRYP水解产物降低了界面张力（20 mN/m），与酪蛋白酸钠（Na-Cas，13 mN/m）相似，但影响较小。动态流变学表明，TRYP水解产物形成了更强的、类似固体的结构。这些结果强调了蛋白酶在提取功能化合物方面优于SFE，增强了虾废料的价值。
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