Guoth J, Gróf J, Menyhárt J
Acta Physiol Hung. 1986;68(2):165-9.
Acidic acetone extract of pregnant sow ovaries was subjected to Sephadex G-25 chromatography. The solution coming from column was analysed for UV absorption, molecular weight, and also for its biological effect on a myometrium strip in vitro. This biodetection system has made it possible continuously to determine the biologically active fractions eluted from the Sephadex G-25 column. The reference materials to calibrate the Sephadex G-25 column were Blue dextran and acetone, while for calibration of the biodetection system, synthetic oxytocin was used. The extract of ovaries of pregnant sows was separated chromatographically into 8 different, biologically active fractions with distinct UV absorption and molecular weight. One of these fractions showed elution characteristics and biological effect similar to those of synthetic oxytocin in the same biodetection system. The results indicated that acidic acetone extract originating from ovaries of pregnant sows is a rich source of biologically active substances with effects on the myometrium strips in pregnancy. Partial identification of oxytocin-like substances in the ovarian extract verified the effectiveness of the biodetection system in the first steps of research to obtain new, biologically active substances from different unpurified extracts.
对怀孕母猪卵巢的酸性丙酮提取物进行葡聚糖凝胶G - 25柱色谱分析。对从柱中流出的溶液进行紫外吸收、分子量分析，并检测其对离体子宫肌条的生物学效应。这种生物检测系统能够连续测定从葡聚糖凝胶G - 25柱上洗脱下来的生物活性组分。校准葡聚糖凝胶G - 25柱的参考物质是蓝色葡聚糖和丙酮，而校准生物检测系统则使用合成催产素。怀孕母猪卵巢提取物经色谱分离为8个不同的具有独特紫外吸收和分子量的生物活性组分。其中一个组分在同一生物检测系统中的洗脱特性和生物学效应与合成催产素相似。结果表明，怀孕母猪卵巢的酸性丙酮提取物富含对妊娠子宫肌条有作用的生物活性物质。卵巢提取物中催产素样物质的部分鉴定证实了该生物检测系统在从不同未纯化提取物中获取新的生物活性物质研究的第一步中的有效性。
