Sánchez-Flores Jesús Enrique, Sandoval-Cabrera Antonio, Alarcón-Valdés Patricia, Santillán-Benítez Jonnathan Guadalupe
Faculty of Chemistry, Autonomous University of The State of Mexico, Toluca, 50120, State of Mexico, México.
Hemato-Oncology High Specialty Laboratory, Childrens Hospital, Maternal and Child Institute of the State of Mexico, Toluca, 50120, Mexico State, México.
Sci Rep. 2025 Mar 26;15(1):10414. doi: 10.1038/s41598-025-87572-w.
DNA serves as the foundation for molecular biology, leading to the development of numerous molecular techniques. Often, these techniques necessitate the separation and visualization of specific DNA regions. Electrophoresis provides a solution for this requirement. However, the purification of DNA from agarose gels presents a significant challenge, both in terms of complexity and cost. Therefore, here we propose two main protocols that are both cost-effective and efficient based on silica columns or freezing follow by alcohol precipitation. In the case of silica column extraction, the gel was partially or completely dissolved, yielding DNA in most situations. In the case of extraction by freezing and precipitation with ethanol, DNA was obtained in only two out of three treatments. A successful bacterial transformation and PCR were achieved confirmed the suitability of the recovered DNA for further applications.
DNA是分子生物学的基础，推动了众多分子技术的发展。通常，这些技术需要对特定的DNA区域进行分离和可视化。电泳为这一需求提供了解决方案。然而，从琼脂糖凝胶中纯化DNA在复杂性和成本方面都面临着重大挑战。因此，在此我们提出两种主要的方案，它们基于硅胶柱或冷冻后乙醇沉淀，既经济又高效。在硅胶柱提取的情况下，凝胶部分或完全溶解，在大多数情况下可获得DNA。在冷冻和乙醇沉淀提取的情况下，三次处理中只有两次获得了DNA。成功的细菌转化和PCR证实了回收的DNA适用于进一步的应用。
