McComb R B, Bowers G N
Am J Clin Pathol. 1985 Jul;84(1):67-73. doi: 10.1093/ajcp/84.1.67.
The wide numeric variation of alkaline phosphatase (ALP: EC 3.1.3.1) values reported from clinical laboratories is clearly revealed by the grossly incompatible data found in large interlaboratory surveys. The authors suggest that this unsatisfactory situation is readily correctable by simply expressing all numeric results on a single scale, the International Clinical Enzyme Scale (ICES). ICES for ALP (ALP/ICES) rests upon a well-defined reference system that relates the IFCC Reference Method for ALP to numerous stable primary and secondary ALP reference materials. The authors show by calibrations with ALP/ICES reference materials that raw coefficients of variation of 25-30% due to reagent, temperature, or instrument differences could be reduced to as low as 2%. ICES and the reference system approach automatically unifies the numeric outputs of the working clinical laboratories by relating all measurements and reference materials to one clearly defined international reference method, yet this concept allows many technologic options in the individual laboratory.
大型实验室间调查中发现的数据严重不兼容清楚地揭示了临床实验室报告的碱性磷酸酶（ALP：EC 3.1.3.1）值在数值上的广泛差异。作者认为，通过简单地将所有数值结果以单一尺度——国际临床酶学尺度（ICES）来表示，这种不尽人意的情况很容易得到纠正。碱性磷酸酶的ICES（ALP/ICES）基于一个定义明确的参考系统，该系统将国际临床化学和检验医学联合会（IFCC）的碱性磷酸酶参考方法与众多稳定的一级和二级碱性磷酸酶参考物质联系起来。作者通过使用ALP/ICES参考物质进行校准表明，由于试剂、温度或仪器差异导致的25%-30%的原始变异系数可降低至低至2%。ICES和参考系统方法通过将所有测量值和参考物质与一种明确界定的国际参考方法相关联，自动统一了临床工作实验室的数值输出，然而这一概念允许各个实验室有多种技术选择。
