Yu Bo, Dai Shuying, Cheng Lei, Lu Qirong, Liu Qing, Chen Hongbo
Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, Wuhan Polytechnic University, Wuhan 430023, China.
Int J Mol Sci. 2025 Mar 13;26(6):2596. doi: 10.3390/ijms26062596.
Lysophosphatidic acid (LPA) is a small bioactive phospholipid which plays an important role during embryonic development and promotes developmental potential of in-vitro-produced (IVP) embryos in several species, including sheep and pigs. In bovines, LPA accelerates IVP blastocyst formation through the Hippo/YAP pathway. However, other LPA effects and its potential receptors during bovine embryo development are less clear. In this study, we used enzyme-linked immunosorbent assay (ELISA) to assess the presence of LPA in bovine oviductal fluid and determine cell apoptosis in embryos after LPA stimulation by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and quantitative reverse transcription polymerase chain reaction (qRT-PCR). We further evaluated potential receptors of LPA through molecular docking, RNA-seq data analysis and quantitative RT-PCR. LPA was found to be present in oviductal fluid. An increase in total cell number and a decrease in apoptosis levels were detected in day 7 blastocysts after LPA treatment. Among eight LPA receptors (LPARs), GPR87 and LPAR2 showed the highest affinity with LPA and their transcripts were expressed in embryos after the 16-cell stage in RNA-seq and qRT-PCR analysis. However, only the expression of was significantly increased in day 6 blastocysts after LPA stimulation, indicating its potential role in LPA-mediated signaling pathways. Our data highlight the positive effects of LPA on embryos and enrich information of related signaling mediators of LPA during embryonic development.
溶血磷脂酸（LPA）是一种小的生物活性磷脂，在胚胎发育过程中发挥重要作用，并能促进包括绵羊和猪在内的多个物种体外生产（IVP）胚胎的发育潜能。在牛中，LPA通过Hippo/YAP信号通路加速IVP囊胚的形成。然而，在牛胚胎发育过程中，LPA的其他作用及其潜在受体尚不清楚。在本研究中，我们使用酶联免疫吸附测定（ELISA）评估牛输卵管液中LPA的存在，并通过末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）测定和定量逆转录聚合酶链反应（qRT-PCR）确定LPA刺激后胚胎中的细胞凋亡。我们通过分子对接、RNA测序数据分析和定量RT-PCR进一步评估了LPA的潜在受体。结果发现输卵管液中存在LPA。LPA处理后第7天的囊胚中检测到总细胞数增加和凋亡水平降低。在8种LPA受体（LPARs）中，GPR87和LPAR2与LPA的亲和力最高，并且在RNA测序和qRT-PCR分析中，它们的转录本在16细胞期后的胚胎中表达。然而，在LPA刺激后第6天的囊胚中，只有 的表达显著增加，表明其在LPA介导的信号通路中的潜在作用。我们的数据突出了LPA对胚胎的积极作用，并丰富了胚胎发育过程中LPA相关信号介质的信息。
