Li Shoujie, Zhang Weiping, Si Can, Chen Jing, Huang Yuhan, Li Muyi, Liang Hanzhi, Duan Jun, He Chunmei
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Int J Mol Sci. 2025 Mar 16;26(6):2671. doi: 10.3390/ijms26062671.
The Dof gene family represents a class of plant-specific transcription factors that play crucial regulatory roles in various biological processes, including plant growth, development, and responses to abiotic stress. However, genome-wide identification and functional characterization of the Dof gene family remain unexplored in . In this study, we performed a genome-wide identification and functional analysis of the DoDof gene family. A total of 28 Dof family members were identified and named based on genome annotation data. Phylogenetic analysis classified these genes into four major groups (A-D) and further subdivided them into nine subfamilies. Gene structure analysis revealed that most lack introns, with no distinct specificity observed among different subfamilies and considerable diversity within the same subfamily. Sequence alignment analysis demonstrated that all DoDof proteins contain a conserved Dof domain consisting of 52 amino acids, which includes a C2-C2 zinc finger motif and a DNA-binding domain. MEME analysis revealed that the conserved motif composition exhibits a certain degree of conservation among DoDof proteins, but significant differences exist across subfamilies. Expression pattern analysis demonstrated that have exhibited diverse expression profiles across different developmental stages, tissues, and under abiotic stresses (such as low temperature, salinity, and drought) in , suggesting their potential roles in plant development and stress responses. Subcellular localization analysis indicated that DoDof15, DoDof22, and DoDof24 are localized exclusively in the nucleus. Yeast one-hybrid assays revealed that DoDof22 binds to the promoter of the ABA receptor , while DoDof15 and DoDof24 bind to the promoter of the bHLH transcription factor . These results suggest that DoDof proteins may regulate the growth, development, and stress response processes of by binding to the promoters of target genes. This study provides critical insights into the functional roles of Dof transcription factors in Orchidaceae family and establishes a theoretical foundation for molecular breeding and stress resistance improvement in .
Dof基因家族代表一类植物特有的转录因子，在包括植物生长、发育以及对非生物胁迫的响应等各种生物学过程中发挥着关键的调控作用。然而，Dof基因家族的全基因组鉴定和功能表征在[具体物种]中仍未得到探索。在本研究中，我们对[具体物种]的Dof基因家族进行了全基因组鉴定和功能分析。基于基因组注释数据，共鉴定出28个Dof家族成员并进行了命名。系统发育分析将这些基因分为四个主要组（A - D），并进一步细分为九个亚家族。基因结构分析表明，大多数[具体物种的Dof基因]缺乏内含子，不同亚家族之间未观察到明显的特异性，同一亚家族内存在相当大的多样性。序列比对分析表明，所有[具体物种的Dof蛋白]都包含一个由52个氨基酸组成的保守Dof结构域，其中包括一个C2 - C2锌指基序和一个DNA结合结构域。MEME分析表明，保守基序组成在[具体物种的Dof蛋白]中表现出一定程度的保守性，但不同亚家族之间存在显著差异。表达模式分析表明，[具体物种的Dof基因]在不同发育阶段、组织以及非生物胁迫（如低温、盐度和干旱）下呈现出多样的表达谱，表明它们在植物发育和胁迫响应中的潜在作用。亚细胞定位分析表明，[具体物种的]Dof15、Dof22和Dof24仅定位于细胞核。酵母单杂交试验表明，Dof22与ABA受体[具体基因名称]的启动子结合，而Dof15和Dof24与bHLH转录因子[具体基因名称]的启动子结合。这些结果表明，[具体物种的]Dof蛋白可能通过与靶基因的启动子结合来调节[具体物种]的生长、发育和胁迫响应过程。本研究为兰科植物中Dof转录因子的功能作用提供了重要见解，并为[具体物种]的分子育种和抗逆性改良奠定了理论基础。
