Yu Xinhai, Yue Weijie, Jia Xinyue, Zeng Hua, Liu Yanxi, Xu Miao, Wu Ming, Guo Liquan
College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
Jilin Institute of Biology, Changchun 130012, China.
Int J Mol Sci. 2025 Mar 17;26(6):2677. doi: 10.3390/ijms26062677.
The key flavonoid biosynthesis-related genes and their molecular features in rice have not been comprehensively and systematically characterized. In this study, we investigated the glumes of OsCSN1 mutants and OsCSN2 mutants and found the changes in the total flavonoid contents of the OsCSN2 mutants to be more pronounced than those of the OsCSN1 mutants and the changes in the anthocyanin contents of the OsCSN1 mutants to be more pronounced than those of the OsCSN2 mutants. In addition, key genes related to flavonoid synthesis, OsCHI, showed a more pronounced up-regulation trend, and the OsDFR gene, which encodes a precursor enzyme for anthocyanin synthesis, showed a clear down-regulation trend. And yeast two-hybrid experiments showed that OsCSN1 and OsCSN2 had the ability to interact with OsCUL4. In summary, OsCSN1 and OsCSN2 may regulate the metabolism of flavonoids in rice through CUL4-based E3 ligase, and the two subunits play different roles, laying a foundation for the study of the mechanism of flavonoid metabolism in monocotyledonous plants.
水稻中与类黄酮生物合成相关的关键基因及其分子特征尚未得到全面系统的表征。在本研究中，我们对OsCSN1突变体和OsCSN2突变体的颖壳进行了研究，发现OsCSN2突变体总黄酮含量的变化比OsCSN1突变体更明显，而OsCSN1突变体花青素含量的变化比OsCSN2突变体更明显。此外，类黄酮合成相关关键基因OsCHI呈现出更明显的上调趋势，而编码花青素合成前体酶的OsDFR基因则呈现出明显的下调趋势。酵母双杂交实验表明，OsCSN1和OsCSN2具有与OsCUL4相互作用的能力。综上所述，OsCSN1和OsCSN2可能通过基于CUL4的E3连接酶调节水稻中类黄酮的代谢，且这两个亚基发挥不同作用，为单子叶植物类黄酮代谢机制的研究奠定了基础。
