Huang Yu, Liu Xiaochen, Feng Tingting, Wang Xiaohua
College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China.
School of Pharmacy, Guilin Medical University, Guilin 541199, China.
Int J Mol Sci. 2025 Mar 18;26(6):2714. doi: 10.3390/ijms26062714.
We developed a fluorescence aptamer sensing method based on gold nanoparticles and graphene quantum dots for the rapid detection of imidacloprid residues in Chinese herbal medicines. In the absence of imidacloprid, gold nanoparticles are dispersed in the solution and effectively quench the fluorescence intensity of the quantum dots due to the protective effect of the aptamer. Because of the aptamer's specific recognition of imidacloprid, a complex forms between the two compounds, and the gold nanoparticles are no longer protected by the aptamer and can aggregate. Consequently, the fluorescence intensity of the graphene quantum dots remain unquenched, resulting in fluorescence recovery. Under optimal conditions, the fluorescence intensity showed a good linear relationship with the imidacloprid concentration in the range of 100-3 × 10 ng/mL. The correlation coefficient was 0.9914, and the detection limit was 52.42 ng/mL. The recoveries of imidacloprid in the yam, matrine, and aloe leaf were 92.27-101.7%, and the relative standard deviation was 0.45-4.14%. This method has potential field applications for rapid quantitative analysis of imidacloprid residue.
我们开发了一种基于金纳米粒子和石墨烯量子点的荧光适配体传感方法，用于快速检测中草药中的吡虫啉残留。在没有吡虫啉的情况下，金纳米粒子分散在溶液中，由于适配体的保护作用，有效地猝灭了量子点的荧光强度。由于适配体对吡虫啉的特异性识别，两种化合物之间形成复合物，金纳米粒子不再受到适配体的保护而发生聚集。因此，石墨烯量子点的荧光强度不再被猝灭，导致荧光恢复。在最佳条件下，荧光强度与吡虫啉浓度在100 - 3×10 ng/mL范围内呈良好的线性关系。相关系数为0.9914，检测限为52.42 ng/mL。吡虫啉在山药、苦参和芦荟叶中的回收率为92.27 - 101.7%，相对标准偏差为0.45 - 4.14%。该方法在吡虫啉残留快速定量分析方面具有潜在的现场应用价值。
