新型生物相容性氰基丙烯酸酯和聚乳酸止血贴片：一项体内概念验证研究。

New Biocompatible Cyanoacrylate and Polylactic Acid Hemostatic Patch: An In Vivo Proof of Concept Study.

作者信息

Ilie-Ene Alexandru, Tosa Victor Petru, Gherman Luciana-Madalina, Pop Raluca Maria, Hantig Lorena-Maria, Popa Catalin Ovidiu, Dindelegan George Calin

机构信息

Department of Surgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania.

Materials Science and Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania.

出版信息

Materials (Basel). 2025 Mar 13;18(6):1271. doi: 10.3390/ma18061271.

DOI:10.3390/ma18061271

PMID:40141554

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11943873/

Abstract

BACKGROUND

Traumatic injuries cause millions of deaths annually, with intra-abdominal hemorrhage as a leading cause. Achieving hemostasis remains challenging. This study evaluates the hemostatic properties of a novel biocompatible cyanoacrylate-polylactic acid patch.

METHODS

Thirty-six male Wistar rats were randomized into three groups (control, study, and TachoSil) and underwent a standardized liver resection. The hemostasis times were recorded, and blood samples were drawn to assess hemoglobin, hematocrit, and cytokine levels (IL-6 and TNF-α) using validated assays.

RESULTS

In the study group, a median time to hemostasis of 94 s was recorded, compared with 256 s for electrocautery ( < 0.001) and 120 s for TachoSil ( = 0.010). Hemoglobin levels significantly decreased postoperatively in all groups, with the TachoSil group showing the lowest median value (from 15.4 g/dL to 11.9 g/dL, = 0.006). Furthermore, only the TachoSil group exhibited a marked inflammatory response with a significant rise in IL-6 (from 15.6 to 26.8 pg/mL, = 0.04) and TNF-α (from 9.8 to 28.6 pg/mL, = 0.01).

CONCLUSIONS

The results demonstrated that the novel cyanoacrylate-polylactic acid patch achieved rapid hemostasis and was associated with reduced inflammatory responses compared with both electrocautery and TachoSil. These findings suggest that this hybrid material may offer a safer and more effective hemostatic alternative.

摘要

背景

创伤性损伤每年导致数百万人死亡，腹腔内出血是主要原因之一。实现止血仍然具有挑战性。本研究评估了一种新型生物相容性氰基丙烯酸酯-聚乳酸贴片的止血特性。

方法

将36只雄性Wistar大鼠随机分为三组（对照组、研究组和速即纱组），并进行标准化肝切除术。记录止血时间，并采集血样，使用经过验证的检测方法评估血红蛋白、血细胞比容和细胞因子水平（IL-6和TNF-α）。

结果

研究组的中位止血时间为94秒，电灼组为256秒（<0.001），速即纱组为120秒（=0.010）。所有组术后血红蛋白水平均显著下降，速即纱组的中位值最低（从15.4 g/dL降至11.9 g/dL，=0.006）。此外，只有速即纱组表现出明显的炎症反应，IL-6（从15.6 pg/mL升至26.8 pg/mL，=0.04）和TNF-α（从9.8 pg/mL升至28.6 pg/mL，=0.01）显著升高。