Kang Yi, Li Chengzhi, Yu Xiaozhang
College of Environmental Science & Engineering, Guilin University of Technology, Guilin 541004, China.
Life (Basel). 2025 Mar 18;15(3):486. doi: 10.3390/life15030486.
Plants experience various abiotic stresses, among which pollutant stress is one of the most damaging, threatening plant productivity and survival. Thiocyanate (SCN), a recalcitrant byproduct of industrial processes, poses escalating threats to agroecosystems by disrupting plant hormonal homeostasis, which is critical for stress adaptation. Here, we dissect the regulatory interplay of phytohormones in rice ( L.) under SCN stress (4.80-124.0 mg SCN/L) through integrated transcriptomic and metabolomic profiling. Quantitative hormonal assays revealed dose- and tissue-specific perturbations in phytohormone homeostasis, with shoots exhibiting higher sensitivity than roots. Transcriptomic analysis revealed that a number of differentially expressed genes (DEGs) mapped in different phytohormone pathways in SCN-treated rice seedlings, and their transcript abundances are tissue-specific. To identify the phytohormones governing rice's sensitivity to SCN stress, we developed a Total Hormonal Sensitivity Index () through an integrative multivariate framework, which combines Modified Variable Importance in Projection () scores to quantify hormonal fluctuations and Total Weighted Contribution Scores () at the gene-level from hormonal pathways. This study establishes a system-level understanding of how phytohormonal crosstalk mediates rice's adaptation to SCN stress, providing biomarkers for phytoremediation strategies in contaminated paddies.
植物会遭受各种非生物胁迫，其中污染物胁迫是最具破坏性的胁迫之一，威胁着植物的生产力和生存。硫氰酸盐（SCN）是工业过程中一种难降解的副产物，通过破坏对胁迫适应至关重要的植物激素稳态，对农业生态系统构成日益严重的威胁。在此，我们通过整合转录组学和代谢组学分析，剖析了水稻在SCN胁迫（4.80 - 124.0 mg SCN/L）下植物激素的调控相互作用。定量激素测定揭示了植物激素稳态中剂量和组织特异性的扰动，地上部分比根表现出更高的敏感性。转录组分析表明，在经SCN处理的水稻幼苗中，许多差异表达基因（DEG）映射到不同的植物激素途径，并且它们的转录丰度具有组织特异性。为了确定控制水稻对SCN胁迫敏感性的植物激素，我们通过一个综合多变量框架开发了一个总激素敏感性指数（），该框架结合了投影中修正的变量重要性（）得分以量化激素波动以及激素途径在基因水平的总加权贡献得分（）。本研究建立了对植物激素相互作用如何介导水稻对SCN胁迫适应的系统水平理解，为污染稻田的植物修复策略提供了生物标志物。
