The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004, People's Republic of China.
Environ Sci Pollut Res Int. 2019 Apr;26(12):11696-11709. doi: 10.1007/s11356-019-04544-0. Epub 2019 Feb 26.
Effective concentrations of potassium thiocyanate (KSCN) to rice seedlings were experimentally determined using relative growth rate as a sensitive endpoint. Agilent 44-K rice microarray was used to profile the molecular responses of rice seedlings exposed to thiocyanate ion (SCN) at three different effective concentrations (EC, EC, and EC). A total of 18,498 known genes were collected from SCN-treated rice microarray analysis. Out of all, 1603, 1882, and 5085 differentially expressed genes (DEGs) were observed at EC, EC, and EC concentrations, respectively. More upregulated/downregulated DEGs were detected in shoots than in roots after SCN exposure. Gene functions and pathway enrichment analysis of DEGs indicated that different effective concentrations of SCN resulted in multiple enriched GO categories and KEGG pathways and outcomes were quite tissue-specific. Different regulations and adaptations of gene expression in molecular function (MF), biological process (BP), and cellular components (CC) were observed in rice tissues at different effective concentrations of SCN, suggesting their different responsive and adaptive strategies. Information collected here presents a detailed description of SCN-induced alternations of gene expression in rice seedlings and provide valuable information for further searching specific genes participating in transportation, phytotoxic responses, and detoxification of SCN in rice seedlings.
采用相对生长率作为敏感终点，实验确定了硫氰酸钾（KSCN）对水稻幼苗的有效浓度。使用安捷伦 44-K 水稻微阵列分析了水稻幼苗在三种不同有效浓度（EC、EC 和 EC）下暴露于硫氰酸根离子（SCN）时的分子反应。从 SCN 处理的水稻微阵列分析中收集了总共 18498 个已知基因。在 EC、EC 和 EC 浓度下，分别观察到 1603、1882 和 5085 个差异表达基因（DEG）。与 SCN 暴露后在根部相比，更多的上调/下调 DEG 在地上部中检测到。DEG 的基因功能和途径富集分析表明，不同有效浓度的 SCN 导致多个 GO 类别和 KEGG 途径富集，且结果具有组织特异性。在不同有效浓度的 SCN 作用下，水稻组织中观察到分子功能（MF）、生物过程（BP）和细胞成分（CC）的基因表达有不同的调控和适应，表明它们有不同的响应和适应策略。这里收集的信息详细描述了 SCN 对水稻幼苗中基因表达的诱导变化，并为进一步搜索参与 SCN 在水稻幼苗中运输、植物毒性反应和解毒的特定基因提供了有价值的信息。
