Hanif Novriyandi, Miftah Jihan Azmi, Yanti Henny Dwi, Oluwabusola Emmanuel Tope, Zahra Vira Amanda, Salleh Nurul Farhana, Kundukad Binu, Tan Lik Tong, Voogd Nicole J de, Rachmania Nisa, Jaspars Marcel, Kjelleberg Staffan, Noviendri Dedi, Murni Anggia, Tanaka Junichi
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia.
Tropical Biopharmaca Research Center, IPB University, Bogor 16128, Indonesia.
Molecules. 2025 Mar 7;30(6):1202. doi: 10.3390/molecules30061202.
Microorganisms play a significant role in biofouling and biocorrosion within the maritime industry. Addressing these challenges requires an innovative and integrated approach utilizing marine natural products with beneficial properties. A comprehensive screening of 173 non-toxic EtOAc and H₂O extracts derived from diverse marine organisms collected in Indonesian waters was conducted using a robust panel of assays. These included antimicrobial tests and classical biosurfactant assays (drop collapse and oil displacement), as well as anti-quorum-sensing (QS) and anti-biofilm assays. These screening efforts identified five active extracts with promising activities. Among these, EtOAc extracts of the marine tunicate cf. (0159-22e) and the marine sponge (0194-24c) demonstrated significant anti-biofouling activity against and anti-biocorrosion performance (mpy 10.70 ± 0.70 for cf. ; 7.87 ± 0.86 for 13.60 ± 1.70 for positive control Tetracorr CI-2915). Further chemical analyses of the active extracts, including LC-HR-MS/MS, MS-based molecular networking, and chemoinformatics, revealed the presence of both known and new bioactive compounds. These included tambjamines and polybrominated diphenyl ethers (PBDEs), which are likely contributors to the observed bioactivities. Subsequent investigations uncovered new anti-QS and anti-biofilm properties in synthetic and natural PBDEs - previously derived from . Among these, exhibited the most potent anti-QS activity, with an IC value of 15 µM, while significantly reduced biofilm formation at a concentration of 1 µM. This study highlights the potential of marine-derived compounds in addressing biofouling and biocorrosion challenges in a sustainable and effective manner.
微生物在海洋工业中的生物污损和生物腐蚀过程中发挥着重要作用。应对这些挑战需要采用创新的综合方法，利用具有有益特性的海洋天然产物。我们使用一系列强大的检测方法，对从印度尼西亚海域采集的173种来自不同海洋生物的无毒乙酸乙酯提取物和水提取物进行了全面筛选。这些检测包括抗菌测试、经典生物表面活性剂检测（液滴坍塌和油置换），以及群体感应（QS）抑制和生物膜抑制检测。这些筛选工作鉴定出了五种具有潜在活性的提取物。其中，海洋被囊动物cf. （0159 - 22e）和海洋海绵（0194 - 24c）的乙酸乙酯提取物对 和 表现出显著的抗生物污损活性以及抗生物腐蚀性能（cf. 的点蚀速率为10.70 ± 0.70密耳/年； 的点蚀速率为7.87 ± 0.86密耳/年；阳性对照Tetracorr CI - 2915的点蚀速率为13.60 ± 1.70密耳/年）。对活性提取物进行的进一步化学分析，包括液相色谱 - 高分辨质谱/质谱联用（LC - HR - MS/MS）、基于质谱的分子网络分析和化学信息学分析，揭示了已知和新的生物活性化合物的存在。这些化合物包括 tambjamines 和多溴二苯醚（PBDEs），它们可能是观察到的生物活性的贡献者。随后的研究发现了合成和天然PBDEs（先前从 中提取）具有新的群体感应抑制和生物膜抑制特性。其中， 表现出最有效的群体感应抑制活性，IC50值为15 μM，而 在浓度为1 μM时显著减少生物膜形成。这项研究突出了海洋来源化合物以可持续和有效的方式应对生物污损和生物腐蚀挑战的潜力。
