Goumbri Bertrand W F, Jansen Olivia, Djang'eing'a Roland Marini, Frederich Michel, Semdé Rasmané, Somé Touridomon Issa, Danthine Sabine, Mouithys-Mickalad Ange
Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments, Institut de Recherche en Science de la Santé (IRSS/CNRST), Ouagadougou 7047, Burkina Faso.
Laboratory of Pharmacognosy, Department of Pharmacy, Center of Interdisciplinary Research on Medicines (CIRM), University of Liege, 4000 Liege, Belgium.
Molecules. 2025 Mar 18;30(6):1360. doi: 10.3390/molecules30061360.
Shea butter (SB) is a raw material fat obtained from C.F. Gaertn kernels. We investigated the direct and indirect protective effects of 10 traditional and industrial SBs and their polar extracts on cell-free systems using ABTS and DPPH radical scavenging assays as well as on singlet oxygen (O) produced by Rose Bengal (RB) photosensitization. Their effects against RB-induced HaCaT cell phototoxicity were also explored. A spectrophotometric assay and HPLC were performed to quantify and identify phenolic content, which was between 14.16 and 82.99 ppm pyrogallol equivalent. These variations could be due to the SB origin and extraction process. These polar fractions exhibited moderate DPPH and strong ABTS radical-scavenging activity. By applying the UV-visible technique, we demonstrated that SBs and their phenolic compounds behave as O quenchers in a dose-dependent manner. Moreover, using a UVR-like model after the irradiation of RB, both polar extracts and crude SB exhibited photoprotective effects, highlighting the indirect protective action. In acellular and cellular models, SB and its polar extracts can act as a free radical scavenger against reactive oxygen species and O quenchers. Due to the maximum absorbance of SB at 280 nm and the antioxidant effect of O quenching, SB polar extracts exhibit photoprotective properties.
乳木果油（SB）是从乳油木果核中提取的一种原料脂肪。我们使用ABTS和DPPH自由基清除试验以及对孟加拉玫瑰红（RB）光敏化产生的单线态氧（O），研究了10种传统和工业用乳木果油及其极性提取物对无细胞系统的直接和间接保护作用。还探讨了它们对RB诱导的HaCaT细胞光毒性的影响。进行了分光光度测定和高效液相色谱法以定量和鉴定酚类含量，其含量在14.16至82.99 ppm焦性没食子酸当量之间。这些差异可能归因于乳木果油的来源和提取工艺。这些极性组分表现出适度的DPPH和较强的ABTS自由基清除活性。通过应用紫外可见技术，我们证明了乳木果油及其酚类化合物以剂量依赖的方式作为单线态氧猝灭剂。此外，在RB照射后使用类似紫外线的模型，极性提取物和粗乳木果油均表现出光保护作用，突出了间接保护作用。在无细胞和细胞模型中，乳木果油及其极性提取物可作为针对活性氧和单线态氧猝灭剂的自由基清除剂。由于乳木果油在280 nm处的最大吸光度以及单线态氧猝灭的抗氧化作用，乳木果油极性提取物表现出光保护特性。
