KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
College of Pharmacy and Integrated Research Institute for Drug Development, Dongguk University-Seoul, Goyang‑si, Gyeonggi‑do 10326, Republic of Korea.
Int J Mol Med. 2017 Dec;40(6):1941-1949. doi: 10.3892/ijmm.2017.3188. Epub 2017 Oct 13.
Ultraviolet B (UVB) radiation causes alterations in the skin, such as epidermal thickening, wrinkle formation and inflammation. Therefore, preventing UVB-induced skin damage can promote general health among the human population. Garcinia mangostana L. (mangosteen) is a fruit that has become a popular botanical dietary supplement because of its perceived role in promoting overall health. The present study investigated the photoprotective effects of α-, β-, γ-mangostins and gartanin against UVB radiation using the HaCaT immortalized human keratinocyte cell line as an in vitro model and hairless mice as an in vivo model. UVB radiation increased the expression of matrix metalloproteinase (MMP)‑1 and ‑9 and decreased the mRNA expression levels of involucrin, filaggrin and loricrin in HaCaT cells; however, these changes were attenuated by pretreating the cells with α-, β-, γ-mangostins and gartanin. Among these compounds, α-mangostin exhibited the greatest effects in reducing UVB-induced skin wrinkles, inhibited epidermal thickening in hairless mice in vivo. Exposure to UVB radiation increased the expression of MMPs and pro-inflammatory cytokines and activated mitogen-activated protein kinases in hairless mice, but these changes were attenuated by α-mangostin. The authors suggested that α-mangostin exerts anti-wrinkle and anti-aging properties.
紫外线 B（UVB）辐射会导致皮肤发生变化，如表皮增厚、皱纹形成和炎症。因此，预防 UVB 诱导的皮肤损伤可以促进人类的整体健康。藤黄（山竹）是一种水果，由于其被认为具有促进整体健康的作用，已成为一种流行的植物性膳食补充剂。本研究使用 HaCaT 永生化人角质形成细胞系作为体外模型和无毛小鼠作为体内模型，调查了 α-、β-、γ-倒捻子素和倒捻子素甲对 UVB 辐射的光保护作用。UVB 辐射增加了基质金属蛋白酶（MMP）-1 和 -9 的表达，并降低了 HaCaT 细胞中内披蛋白、丝聚合蛋白和兜甲蛋白的 mRNA 表达水平；然而，这些变化被 α-、β-、γ-倒捻子素和倒捻子素甲预处理细胞所减弱。在这些化合物中，α-倒捻子素在减少 UVB 诱导的皮肤皱纹方面表现出最大的效果，在体内抑制了无毛小鼠的表皮增厚。UVB 辐射增加了 MMPs 和促炎细胞因子的表达，并激活了无毛小鼠中的丝裂原活化蛋白激酶，但这些变化被 α-倒捻子素所减弱。作者认为，α-倒捻子素具有抗皱和抗衰老的特性。
