Lidberg Kenny, Pilheden Sarah, Relloso Ortiz de Uriarte Mikel, Jonsson Ann-Beth
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 106 91 Stockholm, Sweden.
Microorganisms. 2025 Feb 21;13(3):479. doi: 10.3390/microorganisms13030479.
is a human-specific pathogen that colonizes the nasopharyngeal epithelium, which is populated by a dynamic microbiota that includes species. Currently, little is known about the interaction between commensal lactobacilli and pathogenic , emphasizing a need for deeper studies into the molecular interactions between the two bacteria species. This, in turn, could add clinical and therapeutic value to existing treatments against an infection. In this work, we explored how lactobacilli affect the interplay between and host cells. We report that , but not other tested species, efficiently enters pharyngeal cells via caveolin-mediated lipid raft endocytosis and simultaneously enhances the uptake of , as well as uptake of other pathogenic and non-pathogenic microbes. After promoting internalization, then prevented from being released and transcytozed from a confluent cell layer on microporous transwell membranes. Infected cells increased the level of acidic vacuoles and pathogen clearance over time, while lactobacilli survived inside the cells. Taken together, the data suggest a possible route through which the cellular uptake of lactobacilli can increase the uptake of pathogens for destruction.
是一种定殖于鼻咽上皮的人类特异性病原体，鼻咽上皮中存在由多种物种组成的动态微生物群。目前，关于共生乳酸杆菌与致病性之间的相互作用知之甚少，这凸显了深入研究这两种细菌之间分子相互作用的必要性。这反过来又可以为现有的针对感染的治疗方法增添临床和治疗价值。在这项工作中，我们探讨了乳酸杆菌如何影响与宿主细胞之间的相互作用。我们报告称，能够通过小窝蛋白介导的脂筏内吞作用有效进入咽部细胞，同时增强的摄取，以及其他致病和非致病微生物的摄取。在促进内化后，阻止了从微孔Transwell膜上的汇合细胞层释放和跨细胞转运。随着时间的推移，感染细胞增加了酸性液泡水平和病原体清除率，而乳酸杆菌在细胞内存活。综上所述，数据表明乳酸杆菌的细胞摄取可能增加病原体摄取以进行破坏的一条可能途径。
