Fan Xibei, Lv Ning, Quan Zhexue
Microbiome Center, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University, Shanghai 200438, China.
Microorganisms. 2025 Feb 28;13(3):549. doi: 10.3390/microorganisms13030549.
The composition of the human microbiome is a critical health indicator, and culture-independent methodologies have substantially advanced our understanding of human-associated microorganisms. However, precise identification and characterization of microbial strains require culture-based techniques. Recently, the resurgence of culturomics, combined with high-throughput sequencing technology, has reduced the high labor demand of pure culture methods, facilitating a more efficient and comprehensive acquisition of culturable microbial strains. This study employed an integrated approach combining culturomic and high-throughput sequencing to identify culturable microorganisms on the human scalp and in human saliva and feces. Several strains were identified from the scalp, whereas anaerobic microorganisms were dominant in the saliva and fecal samples. Additionally, the study highlighted the beneficial effects of transportation conditions (liquid nitrogen treatment, dry ice transport, and dimethyl sulfoxide [DMSO] buffer) in preserving culturable microorganisms. A robust methodology was developed for the large-scale acquisition of culturable microorganisms with optimized transport conditions that enhance the potential for isolating a greater diversity of culturable strains.
人类微生物组的组成是一个关键的健康指标，且不依赖培养的方法极大地推动了我们对与人类相关微生物的理解。然而，微生物菌株的精确鉴定和表征需要基于培养的技术。最近，文化组学的复兴与高通量测序技术相结合，降低了纯培养方法对劳动力的高需求，有助于更高效、全面地获取可培养的微生物菌株。本研究采用了一种结合文化组学和高通量测序的综合方法，以鉴定人类头皮、唾液和粪便中的可培养微生物。从头皮中鉴定出了几种菌株，而厌氧微生物在唾液和粪便样本中占主导地位。此外，该研究强调了运输条件（液氮处理、干冰运输和二甲基亚砜 [DMSO] 缓冲液）在保存可培养微生物方面的有益作用。开发了一种强大的方法，用于在优化的运输条件下大规模获取可培养微生物，这增强了分离更多样化可培养菌株的潜力。
