Corrias Maria Valeria, Di Marco Eddi, Bonaretti Carola, Squillario Margherita, Amoroso Loredana, Conte Massimo, Ponzoni Mirco, Biassoni Roberto
Laboratory of Experimental Therapies in Oncology, IRCCS Istituto Giannina Gaslini, Via Gerolamo Gaslini 5, 16147 Genova, Italy.
Central Laboratory, IRCCS Istituto Giannina Gaslini, 00161 Genova, Italy.
Microorganisms. 2025 Mar 19;13(3):691. doi: 10.3390/microorganisms13030691.
The fecal microbiomes of 15 infants with neuroblastoma (NB) at the onset of the disease and after standard-of-care therapy have been prospectively analyzed compared to those of age-matched healthy infants. By applying several algorithms to 16S sequencing, we found that the fecal microbiomes of infants with NB at onset were abundant in , including different descendants of After completing therapy, their abundance decreased to a level like that observed in healthy infants. In contrast, the that showed at the onset an abundance like that of healthy infants doubled their amount after treatment. In infants with NB, the beta diversity of the fecal microbiomes was significantly reduced compared to healthy infants and patients at the end of treatment. The Random Forest algorithm and the Reingold-Tilford heat tree showed that had a higher abundance at the onset, which declined after therapy. Picrust2 inferred pathway analysis indicated that the drug treatment was associated with a reduction in the polyamine pathway, highly represented in samples of NB at the onset. In conclusion, the dysbiosis observed in infants with NB at onset changed following standard-of-care treatment. Still, the composition at the end of treatment did not completely resemble that of healthy infants.
与年龄匹配的健康婴儿相比，我们对15例神经母细胞瘤（NB）婴儿在疾病发作时和标准护理治疗后的粪便微生物群进行了前瞻性分析。通过对16S测序应用多种算法，我们发现发病时NB婴儿的粪便微生物群中 含量丰富，包括 的不同后代。完成治疗后，它们的丰度降至健康婴儿中观察到的水平。相比之下，发病时粪便微生物群丰度与健康婴儿相似的 在治疗后数量增加了一倍。在NB婴儿中，与健康婴儿和治疗结束时的患者相比，粪便微生物群的β多样性显著降低。随机森林算法和Reingold-Tilford热树显示， 在发病时丰度较高，治疗后下降。Picrust2推断通路分析表明，药物治疗与多胺通路的减少有关，多胺通路在NB发病时的样本中高度富集。总之，发病时在NB婴儿中观察到的生态失调在标准护理治疗后发生了变化。然而，治疗结束时的组成并不完全类似于健康婴儿。
