Xie Feiran, Zhou Yanzhi, Zhang Fei, Xiao Peihong
International Medical College, Chongqing Medical University, Chongqing 401331, China.
Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China.
Pharmaceutics. 2025 Feb 20;17(3):282. doi: 10.3390/pharmaceutics17030282.
As the last resort and one of the most crucial antibiotics for multidrug-resistant bacteria, carbapenem is considered the best hope for treating bacterial infections. However, the prompt emergence of carbapenemase-producing bacteria (CPB) poses a striking global health threat. Thus, accurate and rapid methods for the detection of carbapenemase are being requested to guide precise diagnosis, appropriate treatment strategies, and antibiotic stewardship. Although genotypic, phenotypic, and biochemical methods are currently used in clinical practice for CPB detection, they each have their problems that cannot commendably meet the need. In recent years, small-molecule probes have made significant progress and breakthroughs in the rapid detection and subtyping of CPB, providing insights and innovative solutions for the ultra-sensitive detection of CPB. In this minireview, some of the advances, namely, chromogenic probes and methods, fluorogenic probes, dual fluorogenic-chromogenic probes, a chemiluminescent probe, and a novel label-free intracellular calorimetric approach, are summarized, appreciated, and discussed. These methods offer high sensitivity, specificity, and accuracy in a short period in clinical settings without the utilization of sophisticated equipment or professional personnel. We hope that this minireview can provide a reference for the development of rapid detection of CPB and eventually contribute to antibiotic resistance management.
作为治疗多重耐药菌的最后手段和最重要的抗生素之一，碳青霉烯类药物被视为治疗细菌感染的最大希望。然而，产碳青霉烯酶细菌（CPB）的迅速出现对全球健康构成了严重威胁。因此，人们迫切需要准确、快速检测碳青霉烯酶的方法，以指导精准诊断、制定恰当的治疗策略及进行抗生素管理。尽管目前临床实践中使用基因分型、表型和生化方法检测CPB，但这些方法各自存在问题，无法令人满意地满足需求。近年来，小分子探针在CPB的快速检测和亚型分析方面取得了重大进展和突破，为CPB的超灵敏检测提供了思路和创新解决方案。在本综述中，将对一些进展进行总结、评价和讨论，这些进展包括显色探针和方法、荧光探针、双荧光-显色探针、化学发光探针以及一种新型无标记细胞内热分析方法。这些方法在临床环境中无需使用复杂设备或专业人员，就能在短时间内提供高灵敏度、特异性和准确性。我们希望本综述能为CPB快速检测技术的发展提供参考，并最终有助于抗生素耐药性管理。
