Schieferecke Adam J, Kuxhausen Ralph Nadia, Schaffer David V
Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.
Viruses. 2025 Mar 14;17(3):414. doi: 10.3390/v17030414.
The delivery of biomolecules to target cells has been a longstanding challenge in biotechnology. DNA viruses naturally evolved the ability to deliver genetic material to cells and modulate cellular processes. As such, they inherently possess requisite characteristics that have led to their extensive study, engineering, and development as biotechnological tools. Here, we overview the application of DNA viruses to biotechnology, with specific implications in basic research, health, biomanufacturing, and agriculture. For each application, we review how an increasing understanding of virology and technological methods to genetically manipulate DNA viruses has enabled advances in these fields. Additionally, we highlight the remaining challenges to unlocking the full biotechnological potential of DNA viral technologies. Finally, we discuss the importance of balancing continued technological progress with ethical and biosafety considerations.
将生物分子递送至靶细胞一直是生物技术领域长期面临的挑战。DNA病毒自然进化出了将遗传物质递送至细胞并调节细胞过程的能力。因此，它们天生具备一些必要特性，这使得它们作为生物技术工具得到了广泛研究、改造和开发。在此，我们概述DNA病毒在生物技术中的应用，及其在基础研究、健康、生物制造和农业方面的具体影响。对于每种应用，我们回顾了对病毒学的深入理解以及对DNA病毒进行基因操作的技术方法是如何推动这些领域取得进展的。此外，我们强调了在释放DNA病毒技术的全部生物技术潜力方面仍然存在的挑战。最后，我们讨论了在持续的技术进步与伦理和生物安全考量之间取得平衡的重要性。
