Li Yuxiao, Zheng Shichao, Li Hongrui, Li Yanhong, Wang Ziliang, Wang Wei, Zheng Bowen, Bai Xishan, Huang Xiangzhong
Key Laboratory of Chemistry in Ethnic Medicinal Resources, Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming, China.
Affiliated Hospital of Yunnan University, Kunming, China.
Nat Prod Res. 2025 Mar 26:1-10. doi: 10.1080/14786419.2025.2484254.
As a Yi medicine for relieving pain, the roots of (Oliv.) Wei Wang are widely used to treat pain-related diseases. However, investigation of its effective analgesic constituents is very poor. In this study, two new clerodane diterpenoids, tinophyllol A () and tinophyllol B (), as well as eight known ones, were isolated from the roots of and identified using the HRESIMS, IR, and NMR data. Meanwhile, their analgesic activities were evaluated using the acetic acid-induced nociception model. The isolated compounds ( and -) demonstrated effective antinociceptive activity. exhibits the best antinociceptive activity, inhibiting 59.2%, with an ED of 26.3 ± 3.2 mg/kg. It also demonstrates clear antinociceptive effects in the other two nociception models induced by hot plate and formalin. Mechanism investigation showed that could exhibit central and peripheral analgesic activity, possibly by modulating the glutamatergic and vanilloid systems (TRPV1).
