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转移至硝酸纤维素膜上用作免疫原的蛋白质。 - Suppr | 超能文献

转移至硝酸纤维素膜上用作免疫原的蛋白质。

Proteins transferred to nitrocellulose for use as immunogens.

作者信息

Knudsen K A

出版信息

Anal Biochem. 1985 Jun;147(2):285-8. doi: 10.1016/0003-2697(85)90273-8.

DOI:10.1016/0003-2697(85)90273-8

Abstract

Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were eluted efficiently from the gel by transfer to nitrocellulose paper. The protein-bearing nitrocellulose was solubilized with dimethyl sulfoxide and used as an immunogen in rabbits. Polyclonal antibodies to the proteins were generated.

摘要

蛋白质通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离，然后通过转移至硝酸纤维素纸上从凝胶中高效洗脱。含蛋白质的硝酸纤维素用二甲基亚砜溶解，并用作家兔的免疫原。产生了针对这些蛋白质的多克隆抗体。

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