Cui Lidan, Liu Baogui, Ling Zhihui, Liu Kehong, Tan Simin, Gong Zhihua, Xiao Wenjun
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Agricultural University, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China.
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Agricultural University, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China.
Food Chem. 2025 Jul 15;480:143935. doi: 10.1016/j.foodchem.2025.143935. Epub 2025 Mar 19.
Epigallocatechin-3-gallate (EGCG), a major catechin in green tea, exhibits potent antioxidant and disease-preventive properties, but its application is limited by poor stability and bioavailability. This study aimed to address these challenges by preparing and characterizing three EGCG-loaded nanoparticles: chitosan-EGCG-tripolyphosphate nanoparticles (CE-NPs), β-cyclodextrin-EGCG (BE-NPs), and EGCG-nanostructured lipid carriers (NE-NPs). BE-NPs exhibited the highest loading performance and retention rate under thermal environment (89.78 % after 10 h at 80 °C). NE-NPs had the highest EGCG stability in alkaline condition (45 % after 4 h at pH 7.4). Compared to free EGCG, all NPs significantly improved in vitro bioaccessibility following incubation in simulated gastrointestinal digestion for 4 h; BE-NPs enhanced oral bioavailability by 1.71 times in vivo. Additionally, CE-NPs and NE-NPs increased the relative abundance of Faecalibaculum, Erysipelotrichaceae, and Bifidobacterium in the colons of Sprague-Dawley rats. These findings suggest that BE-NPs are a promising nano-delivery system for enhancing EGCG stability and bioavailability in healthy organisms.
表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶中的主要儿茶素，具有强大的抗氧化和疾病预防特性，但其应用受到稳定性差和生物利用度低的限制。本研究旨在通过制备和表征三种负载EGCG的纳米颗粒来应对这些挑战：壳聚糖-EGCG-三聚磷酸纳米颗粒（CE-NPs）、β-环糊精-EGCG（BE-NPs）和EGCG-纳米结构脂质载体（NE-NPs）。BE-NPs在热环境下表现出最高的负载性能和保留率（80℃下10小时后为89.78%）。NE-NPs在碱性条件下具有最高的EGCG稳定性（pH 7.4下4小时后为45%）。与游离EGCG相比，所有纳米颗粒在模拟胃肠道消化4小时后，体外生物可及性均显著提高；BE-NPs在体内将口服生物利用度提高了1.71倍。此外，CE-NPs和NE-NPs增加了Sprague-Dawley大鼠结肠中Faecalibaculum、丹毒丝菌科和双歧杆菌的相对丰度。这些发现表明，BE-NPs是一种有前途的纳米递送系统，可提高EGCG在健康生物体中的稳定性和生物利用度。
