Ding Yu, Sha Wenbin, Sun Yunfei, Cheng Yongxu
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquaculture Engineering and Technology Research Centre, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquaculture Engineering and Technology Research Centre, Shanghai Ocean University, Shanghai 201306, China.
Comp Biochem Physiol B Biochem Mol Biol. 2025 Jun-Jul;278:111095. doi: 10.1016/j.cbpb.2025.111095. Epub 2025 Mar 25.
Crayfish (Procambarus clarkii) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of P. clarkii in cold waters.
克氏原螯虾（Procambarus clarkii）养殖受到诸如寒潮和季节波动等气候现象导致的温度骤降的威胁。在本研究中，将克氏原螯虾暴露于从17℃至7℃的温度骤变环境中24小时，以研究急性低温对呼吸代谢、抗氧化剂和代谢组学的影响。结果表明，急性低温显著降低了鳃和血淋巴中丙酮酸激酶、乳酸脱氢酶和琥珀酸脱氢酶的活性，这与无氧和有氧呼吸能力的降低以及耗氧量、氨排泄量和最大代谢率的显著下降有关。肝胰腺和血淋巴中的抗氧化酶在24小时内先升高后降低。代谢组学显示，甘油磷脂代谢和糖基磷脂酰肌醇锚定生物合成途径对急性低温有反应，甘油磷脂是差异表达最显著的代谢物。这些结果支持了克氏原螯虾在低温下代谢活性较低的假设。我们的数据为急性低温诱导的生物学变化提供了机制性见解，并可能为冷水环境中克氏原螯虾的养殖提供参考。
