Zhu Xiaochen, Peng Aidi, Zou Yueying, Li Yingdong, Wei Hua, Zheng Xianhu, Zhao Yingying
Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Int J Mol Sci. 2025 Jan 30;26(3):1221. doi: 10.3390/ijms26031221.
The aquaculture industry of red swamp crayfish (RSC), , has grown significantly in recent decades due to increasing market demand. In China, low water temperatures, particularly during overwintering, pose a challenge, hindering the development of the RSC aquaculture industry in northern regions. Understanding the molecular mechanism of RSCs' responses to cold stress could be beneficial for its aquaculture practices. In this study, we exposed RSCs to 4 °C (T4) and 22 °C (T22: control) for 96 h. Transcriptomic and metabolomic analyses of hepatopancreas tissues were performed to identify key genes and metabolites that participate in cold stress response. A total of 787 differentially expressed genes (DEGs) and 198 differentially expressed metabolites (DEMs) were identified between T4 and T22. DEGs were significantly enriched in KEGG pathways related to carbohydrate, lipid, amino acid, and nucleotide metabolism, immunity, and signaling, while DEMs were significantly enriched in pathways associated with lipid and amino acid metabolism and membrane transport. The results indicated that cold stress altered RSCs' metabolism and their innate immune system. This study provides valuable information to increase our understanding of cold stress responses in RSCs.
近几十年来，由于市场需求不断增加，克氏原螯虾的水产养殖业显著发展。在中国，低温，尤其是越冬期间的低温，构成了一项挑战，阻碍了北方地区克氏原螯虾水产养殖业的发展。了解克氏原螯虾对冷应激反应的分子机制可能有助于其水产养殖实践。在本研究中，我们将克氏原螯虾置于4°C（T4）和22°C（T22：对照）环境下96小时。对肝胰腺组织进行转录组学和代谢组学分析，以鉴定参与冷应激反应的关键基因和代谢物。在T4和T22之间共鉴定出787个差异表达基因（DEGs）和198个差异表达代谢物（DEMs）。DEGs在与碳水化合物、脂质、氨基酸和核苷酸代谢、免疫及信号传导相关的KEGG通路中显著富集，而DEMs在与脂质和氨基酸代谢及膜转运相关的通路中显著富集。结果表明，冷应激改变了克氏原螯虾的代谢及其固有免疫系统。本研究提供了有价值的信息，以增进我们对克氏原螯虾冷应激反应的理解。
