Zeng Yulan, Wang Xinyang, Chen Zhonghui, Luo Fang, Qiu Bin, Fu Fengfu, Wang Jian, Lin Zhenyu
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
Affiliated Hospital of Putian University, Putian University, Putian, Fujian 351100, China.
Anal Chem. 2025 Apr 8;97(13):7467-7475. doi: 10.1021/acs.analchem.5c00321. Epub 2025 Mar 27.
Real time detection of hydrogen sulfide (HS) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (CuO NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. CuO reacts with HS to generate CuS. The resulting CuO/CuS heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of HS concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive biomarker detection.
