Padmanabhan R V, Gudapaty R, Liener I E, Schwartz B A, Hoidal J R
Am Rev Respir Dis. 1985 Jul;132(1):164-7. doi: 10.1164/arrd.1985.132.1.164.
To test the feasibility of using liposomes to deliver therapeutic agents to the lungs, the effect of liposome-encapsulated superoxide dismutase (SOD) or catalase on pulmonary oxygen toxicity was studied in rats. The SOD or catalase was encapsulated in negatively changed multilamellar liposomes and administered directly into the trachea of adult rats, which were subsequently exposed to hyperoxia (greater than 95% O2). Response to hyperoxia was examined by studying lung SOD and catalase activities, survival rates, and lung morphology. Rats receiving liposome-encapsulated SOD or catalase showed increased levels of enzyme activities in the lung homogenates compared with those in the control groups after 24 to 72 h of hyperoxic exposure. Elevated enzyme levels in the lungs of rats treated with liposome-encapsulated SOD or catalase were accompanied by a significant improvement in survival rates after 72 h of hyperoxic exposure and less lung injury than in the other control groups.
为了测试使用脂质体将治疗剂递送至肺部的可行性，研究了脂质体包裹的超氧化物歧化酶（SOD）或过氧化氢酶对大鼠肺部氧中毒的影响。将SOD或过氧化氢酶包裹在带负电荷的多层脂质体中，并直接注入成年大鼠的气管，随后将其暴露于高氧环境（氧气含量大于95%）。通过研究肺组织中的SOD和过氧化氢酶活性、存活率以及肺形态来检测对高氧的反应。在高氧暴露24至72小时后，接受脂质体包裹的SOD或过氧化氢酶的大鼠，其肺匀浆中的酶活性水平相较于对照组有所升高。在高氧暴露72小时后，用脂质体包裹的SOD或过氧化氢酶处理的大鼠肺部酶水平升高，同时存活率显著提高，且肺损伤程度比其他对照组轻。
