Luo Jinting, Wang Lei, Yao Youli, Luo Xuan, Zhang Jianbo, Luo Dandan, Tian Tian, Wu Guofang
Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Academy of Animal Science and Veterinary, Qinghai University, Xining, 810016, China.
BMC Vet Res. 2025 Mar 27;21(1):211. doi: 10.1186/s12917-025-04669-0.
Hypoxia can affect the function of the male reproductive system and reduce fertility. Rhodiola polysaccharide (RDP) is the active ingredient of Rhodiola rosea L. and has a positive effect on reproductive cells. However, the mechanism of the effect of RDP on the proliferation of cells under hypoxia is still unclear. The experiment selected porcine Leydig cells (PLCs) as the test object and divided them into three groups: normal group, hypoxia group, and hypoxia + RDP-treated group. Cell viability was detected using CCK8 assay. RNA-Seq technology was used to identify the key genes that influence the effect of RDP on PLCs under hypoxia conditions and to determine their regulatory pathways. Transcriptome sequencing of PLCs from the N and H groups identified 6,794 differentially expressed genes (DEGs), including 3,329 up-regulated genes and 3,465 down-regulated genes. These DEGs were significantly enriched in the cell cycle signaling pathway, indicating that hypoxia mainly affects the cell cycle and inhibits cell proliferation. Furthermore, comparison of the transcriptomes between the H and HR group revealed 285 DEGs, including 137 up-regulated and 148 down-regulated, most of DEGs were found to be enriched in oxidative phosphorylation pathways. RDP inhibits PLCs apoptosis and promotes cell proliferation by up-regulating the expression of CXCL2, JUNB and VCAM1 of the TNF signaling pathway, and VEGFA, SGK2 and SPP1 of the PI3K/AKT signaling pathway. These genes deserve further study as candidate for understanding the role of RDP in alleviating the hypoxia stress.
缺氧会影响男性生殖系统的功能并降低生育能力。红景天多糖（RDP）是红景天的活性成分，对生殖细胞有积极作用。然而，RDP在缺氧条件下对细胞增殖影响的机制仍不清楚。本实验选取猪睾丸间质细胞（PLCs）作为研究对象，将其分为三组：正常组、缺氧组和缺氧+RDP处理组。采用CCK8法检测细胞活力。利用RNA-Seq技术鉴定在缺氧条件下影响RDP对PLCs作用的关键基因，并确定其调控途径。对正常组和缺氧组的PLCs进行转录组测序，共鉴定出6794个差异表达基因（DEGs），其中上调基因3329个，下调基因3465个。这些DEGs在细胞周期信号通路中显著富集，表明缺氧主要影响细胞周期并抑制细胞增殖。此外，比较缺氧组和缺氧+RDP处理组的转录组，发现285个DEGs，其中上调137个，下调148个，大多数DEGs在氧化磷酸化途径中富集。RDP通过上调TNF信号通路的CXCL2、JUNB和VCAM1以及PI3K/AKT信号通路的VEGFA、SGK2和SPP1的表达来抑制PLCs凋亡并促进细胞增殖。这些基因作为理解RDP在减轻缺氧应激中作用的候选基因值得进一步研究。
