Sevimli Murat, Bayram Dilek, Özgöçmen Meltem, Armağan Ilkay, Semerci Sevimli Tuğba
Suleyman Demirel University, Faculty of Medicine, Department of Histology and Embryology, Isparta, Turkey.
Suleyman Demirel University, Faculty of Medicine, Department of Histology and Embryology, Isparta, Turkey.
J Trace Elem Med Biol. 2022 May;71:126958. doi: 10.1016/j.jtemb.2022.126958. Epub 2022 Feb 19.
BACKGROUND/AIM: Colon cancer is one of the most common cancers. Treatment success and survival rates are not high enough with current approaches. Therefore, there is a need to develop new agents and treatment methods. Boric acid is the most frequently observed form of boron. Some epidemiological data suggest that environmental exposure to boric acid reduces the incidence of prostate cancer in men, cervical and lung cancers in women. Experimental studies show, boric acid reduces cell proliferation and stimulates apoptosis in some prostate, melanoma, breast cancer cell lines. In this study, it was investigated whether boric acid could be a new candidate molecule that could be used in the treatment of colon cancer.
The effects of boric acid on human colon adenocarcinoma cell line SW-480 were investigated with BrdU, TUNEL, Caspase-3, and AIF immunohistochemical studies in both 2D and 3D culture systems. In addition, a qRT-PCR study was carried out to determine the expression changes in key genes that take part in apoptosis.
We observed that boric acid suppresses cell proliferation and induces apoptosis both in 2D and 3D culture conditions. In addition, as a result of qRt-PCR studies, it was revealed that the observed apoptotic process was related to the TNF signaling pathway.
Boric acid can be considered as a potential anti-cancer agent candidate for colon cancer treatment.
All data generated or analyzed during this study are included in this published article.
背景/目的：结肠癌是最常见的癌症之一。目前的治疗方法在治疗成功率和生存率方面还不够高。因此，需要开发新的药物和治疗方法。硼酸是最常见的硼的形式。一些流行病学数据表明，环境暴露于硼酸可降低男性前列腺癌、女性宫颈癌和肺癌的发病率。实验研究表明，硼酸可减少某些前列腺癌、黑色素瘤、乳腺癌细胞系中的细胞增殖并刺激细胞凋亡。在本研究中，研究了硼酸是否可能成为可用于治疗结肠癌的新候选分子。
在二维和三维培养系统中，通过BrdU、TUNEL、Caspase-3和AIF免疫组化研究，研究了硼酸对人结肠腺癌细胞系SW-480的影响。此外，进行了qRT-PCR研究以确定参与细胞凋亡的关键基因的表达变化。
我们观察到，在二维和三维培养条件下，硼酸均能抑制细胞增殖并诱导细胞凋亡。此外，qRt-PCR研究结果表明，观察到的凋亡过程与TNF信号通路有关。
硼酸可被视为结肠癌治疗的潜在抗癌药物候选物。
本研究期间生成或分析的所有数据均包含在本发表文章中。
